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To further assess whether HH<\/jats:styled-content>cy enhances MEJ<\/jats:styled-content> communication, this study examined endothelium\u2010dependent attenuation of phenylephrine\u2010induced vasoconstriction (myoendothelial feedback) and key molecules involved.<\/jats:p><\/jats:sec>Methods<\/jats:title>Myoendothelial feedback was examined in isolated mouse mesenteric arteries, after 6\u2010weeks diet\u2010induced HH<\/jats:styled-content>cy, using pressure myography. Gap junction (Cx37, Cx40, Cx43), NOS<\/jats:styled-content> (eNOS<\/jats:styled-content>, nNOS<\/jats:styled-content>, iNOS<\/jats:styled-content>), and potassium channel (IK<\/jats:styled-content>1) protein expression were measured with immunoblots, and connexin mRNA<\/jats:styled-content>s with real\u2010time PCR<\/jats:styled-content>. Contribution of nNOS<\/jats:styled-content>\u00a0+\u00a0iNOS<\/jats:styled-content> to vasomotor responses was assessed using the drug TRIM.<\/jats:p><\/jats:sec>Results<\/jats:title>Myoendothelial feedback was significantly (P<\/jats:italic>\u00a0<\u00a0.05) enhanced in HH<\/jats:styled-content>cy arteries compared to control, coincident with significantly greater Cx37 and IK<\/jats:styled-content>1 protein and Cx37 mRNA<\/jats:styled-content>. Cx43 protein, but not mRNA<\/jats:styled-content>, was significantly less in HH<\/jats:styled-content>cy, and Cx40 was not different. eNOS<\/jats:styled-content> protein was significantly less in HH<\/jats:styled-content>cy. nNOS<\/jats:styled-content> and iNOS<\/jats:styled-content> were not different. TRIM<\/jats:styled-content> had little effect on vasomotor function.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Diet\u2010induced HH<\/jats:styled-content>cy enhanced myoendothelial feedback, and increased Cx37 and IK<\/jats:styled-content>1 expression may contribute. nNOS<\/jats:styled-content> or iNOS<\/jats:styled-content> did not upregulate to compensate for decreased eNOS<\/jats:styled-content>, and they had little involvement in vasomotor function.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12398","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T12:02:39Z","timestamp":1504180959000},"source":"Crossref","is-referenced-by-count":7,"title":["Increased myoendothelial feedback is associated with increased connexin37 and IK<\/scp>1 channel expression in mesenteric arteries of diet\u2010induced hyperhomocysteinemic 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