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Subsequently, application rules have been created to define the<jats:styled-content style=\"fixed-case\">ROI<\/jats:styled-content>in<jats:styled-content style=\"fixed-case\">NVC<\/jats:styled-content>images, through a training images set. The software reliability was assessed through calculation of the<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>between automatic and manual counting, by four independent observers, on the same<jats:styled-content style=\"fixed-case\">NVC<\/jats:styled-content>images.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The following<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>'s were obtained per observer, for the patients with<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c (40 images), the healthy (20 images), and the<jats:styled-content style=\"fixed-case\">PRP<\/jats:styled-content>subgroups (20 images), respectively: 0.94, 0.81, and 0.62 (observer 1); 0.94, 0.91, and 0.67 (observer 2); 0.88, 0.56, and 0.64 (observer 3); and 0.88, 0.85, and 0.85 (observer 4).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The validation of an automated software for measuring absolute nailfold capillary number and density in<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c was achieved. The integration into the pre\u2010existing imaging software should make the assessment of the capillary number in<jats:styled-content style=\"fixed-case\">NVC<\/jats:styled-content>easier, quicker, and standardized.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12447","type":"journal-article","created":{"date-parts":[[2018,3,12]],"date-time":"2018-03-12T07:32:39Z","timestamp":1520839959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Automated assessment of absolute nailfold capillary number on videocapillaroscopic images: Proof of principle and validation in systemic sclerosis"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5396-0932","authenticated-orcid":false,"given":"Maurizio","family":"Cutolo","sequence":"first","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia C.","family":"Trombetta","sequence":"additional","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Melsens","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Ghent University Hospital Ghent Belgium"},{"name":"Department of Internal Medicine Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Pizzorni","sequence":"additional","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Sulli","sequence":"additional","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Ruaro","sequence":"additional","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Paolino","sequence":"additional","affiliation":[{"name":"Research Laboratory and Academic Division of Clinical Rheumatology Department of Internal Medicine University of Genoa San Martino Polyclinic Hospital Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Deschepper","sequence":"additional","affiliation":[{"name":"Biostatistics Unit Department of Public Health Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Ghent University Hospital Ghent Belgium"},{"name":"Department of Internal Medicine Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1919.sp001839"},{"key":"e_1_2_8_3_1","first-page":"243","article-title":"Capillary density in developing and healing tuberculous lesions produced by BCG in rabbits. 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