{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:36:25Z","timestamp":1792467385594,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,5,12]],"date-time":"2018-05-12T00:00:00Z","timestamp":1526083200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["17\u201004\u201000157\\17"],"award-info":[{"award-number":["17\u201004\u201000157\\17"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> characterizes penetration of red blood cells inside glycocalyx and its thickness can have profound impact on microcirculation and other vascular parameters. The goal of our study was to reliably quantify <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> and assess its potential use as a new marker of cardiovascular pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 208 patients (123 men and 85 women from 40 to 65\u00a0years of age) with various grades of cardiovascular <jats:styled-content style=\"fixed-case\">SCORE<\/jats:styled-content> risk index and IHD. <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> was quantified by sidestream dark field capillaroscopy with green light excitation. Cutaneous microcirculation was evaluated with laser Doppler fluorometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Elevated <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> values over 2\u00a0mm were associated with morphological and functional lesions of arterial wall and microcirculation and lowered levels of ApoA1 lipoprotein. Moreover, elevated <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> values were associated with 2.07\u2010fold increase in prevalence of cerebral atherosclerosis (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.015) and 2.42\u2010fold increase in prevalence of IHD (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.024). Increase in <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> was associated with elevated systolic blood pressure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Thus, <jats:styled-content style=\"fixed-case\">PBR<\/jats:styled-content> can be considered a new highly reproducible and promising marker candidate for non\u2010invasive diagnostics of IHD and cerebral atherosclerosis suggesting important role of microcirculation in development and progression of cardiovascular diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12454","type":"journal-article","created":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T16:21:01Z","timestamp":1522686061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Increase in perfused boundary region of endothelial glycocalyx is associated with higher prevalence of ischemic heart disease and lesions of microcirculation and vascular wall"],"prefix":"10.1111","volume":"25","author":[{"given":"Alexander Y.","family":"Gorshkov","sequence":"first","affiliation":[{"name":"National Research Center for Preventive Medicine Ministry of Healthcare of Russian Federation  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Klimushina","sequence":"additional","affiliation":[{"name":"National Research Center for Preventive Medicine Ministry of Healthcare of Russian Federation  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei A.","family":"Boytsov","sequence":"additional","affiliation":[{"name":"National Research Center for Preventive Medicine Ministry of Healthcare of Russian Federation  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Y.","family":"Kots","sequence":"additional","affiliation":[{"name":"School of Medicine and Health Sciences The George Washington University  Washington District of Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6108-3538","authenticated-orcid":false,"given":"Nadezhda G.","family":"Gumanova","sequence":"additional","affiliation":[{"name":"National Research Center for Preventive Medicine Ministry of Healthcare of Russian Federation  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1485-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000131515.03336.f8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.02.092"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e328321b587"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.1.H285"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000104871"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.3.581"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40560-016-0182-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096477"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011121181"},{"issue":"4","key":"e_1_2_9_12_1","first-page":"1","article-title":"National recommendations about diagnostics and treatment of stable angina","volume":"7","year":"2008","journal-title":"Cardiovasc Ther Prev"},{"key":"e_1_2_9_13_1","first-page":"33","article-title":"Dark field microscopy for the endothelial glycocalyx study: first Russian experience; 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