{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:27:39Z","timestamp":1791350859650,"version":"4.3.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 106\u20102314\u2010B\u2010075\u2010015\u2010MY3"],"award-info":[{"award-number":["MOST 106\u20102314\u2010B\u2010075\u2010015\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to determine whether high\u2010dose nitroglycerin, a nitric oxide donor, preserves erythrocyte deformability during cardiopulmonary bypass and examines the signaling pathway of nitric oxide in erythrocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a randomized and controlled fashion, forty\u2010two patients undergoing cardiac surgery with hypothermic cardiopulmonary bypass were allocated to high\u2010dose (N\u00a0=\u00a021) and low\u2010dose groups (N\u00a0=\u00a021). During rewarming period, patients were given intravenous nitroglycerin with an infusion rate 5 and 1\u00a0\u00b5g\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7min<jats:sup>\u22121<\/jats:sup>in high\u2010dose and low\u2010dose groups, respectively. Tyrosine phosphorylation level of non\u2010muscle myosin IIA in erythrocyte membrane was used as an index of erythrocyte deformability and analyzed using immunoblotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tyrosine phosphorylation of non\u2010muscle myosin IIA was significantly enhanced after bypass in high\u2010dose group (3.729\u00a0\u00b1\u00a01.700 folds,<jats:italic>P<\/jats:italic>\u00a0=\u00a0.011) but not low\u2010dose group (1.545\u00a0\u00b1\u00a00.595 folds,<jats:italic>P<\/jats:italic>\u00a0=\u00a0.076). Phosphorylation of aquaporin 1, vasodilator\u2010stimulated phosphoprotein, and focal adhesion kinase in erythrocyte membrane was also upregulated in high\u2010dose group after bypass. Besides, plasma nitric oxide level was highly correlated with fold change of non\u2010muscle myosin IIA phosphorylation (Pearson's correlation coefficient .871).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>High\u2010dose nitroglycerin administered during cardiopulmonary bypass improves erythrocyte deformability through activating phosphorylation of aquaporin 1, vasodilator\u2010stimulated phosphoprotein, and focal adhesion kinase in erythrocytes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12608","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:25:24Z","timestamp":1580257524000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["High\u2010dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass"],"prefix":"10.1111","volume":"27","author":[{"given":"Ying\u2010Hsuan","family":"Tai","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Taipei Veterans General Hospital Taipei Taiwan"},{"name":"School of Medicine National Yang\u2010Ming University Taipei Taiwan"},{"name":"Department of Anesthesiology Shuang Ho Hospital Taipei Medical University New Taipei City Taiwan"},{"name":"Department of Anesthesiology School of Medicine College of Medicine Taipei Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Hsiang","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Taipei Veterans General Hospital Taipei Taiwan"},{"name":"Graduate Institute of Life Sciences National Defense Medical Center Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiang\u2010Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Taipei Veterans General Hospital Taipei Taiwan"},{"name":"School of Medicine National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Man","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Taipei Veterans General Hospital Taipei Taiwan"},{"name":"School of Medicine National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei\u2010Yung","family":"Tsou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Taipei Veterans General Hospital Taipei Taiwan"},{"name":"School of Medicine National Yang\u2010Ming University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Hsiung","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine National Yang\u2010Ming University Taipei Taiwan"},{"name":"Division of Cardiovascular Surgery 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