{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:09:52Z","timestamp":1761664192472,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T00:00:00Z","timestamp":1612483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81473571"],"award-info":[{"award-number":["81473571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the effect of Yiqifumai injection (YQFM), a compound Chinese medicine, and its main active ingredients on lipopolysaccharide (LPS)\u2010induced microvascular disturbance in mesentery and ileum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats were infused with LPS (5\u00a0mg\/kg\/h) for 90\u00a0min. Thirty minutes after initiation of LPS administration, YQFM (160\u00a0mg\/kg\/h), Rb1 (5\u00a0mg\/kg\/h), Sch (2.5\u00a0mg\/kg\/h), or Rb1+Sch (5\u00a0mg\/kg\/h\u00a0+\u00a02.5\u00a0mg\/kg\/h) was infused until 90\u00a0min. Human umbilical vein endothelial cells (HUVECs) were incubated with LPS (100\u00a0ng\/ml) for 90\u00a0min. YQFM (1\u00a0mg\/ml), Rb1 (100\u00a0\u00b5M), Sch (100\u00a0\u00b5M), or Rb1+Sch (200\u00a0\u00b5M) was added 30\u00a0min after initiation of LPS stimulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Yiqifumai injection and Rb1+Sch inhibited mesenteric venule hyperpermeability, suppressed microvillar erosion and submucosal edema, and protected claudin\u20105 from downregulation and interleukin\u20101\u03b2 from upregulation in ileal tissues after LPS. Study in HUVECs confirmed the effect of YQFM and Rb1+Sch on JAM\u20101 after LPS and revealed a similar effect on other junction proteins. Moreover, YQFM and Rb1+Sch attenuated the dysfunctional energy metabolism and the activation of TLR\u20104\/Src\/NF\u2010\u03baB signaling with Rb1 and Sch being partially effective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results demonstrated the beneficial effect of post\u2010treatment with YQFM, which is attributable to its main ingredient Rb1 and Sch, and likely mediated by targeting TLR\u20104\/Src\/NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12680","type":"journal-article","created":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T07:49:04Z","timestamp":1611474544000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Post\u2010treatment with yiqifumai injection and its main ingredients attenuates lipopolysaccharide\u2010induced microvascular disturbance in mesentery and 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