{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:14:56Z","timestamp":1790946896904,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T00:00:00Z","timestamp":1664668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>NLRP3 inflammasome mediates myocardial ischemia\/reperfusion (MI\/R) injury and diabetic vascular endothelia dysfunction. However, the role of NLRP3 inflammasome in MI\/R injury with diabetes has not been fully described. Irisin plays an important role in anti\u2010inflammation and improves endothelial function in type 2 diabetes. The current study aimed to investigate the effect of irisin on regulating NLRP3 inflammasome activation in diabetic vascular endothelia dysfunction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cardiac microvascular endothelial cells (CMECs) were cultured and subjected to high glucose\/high fat (HG\/HF) receiving hypoxia\/reoxygenation (H\/R) with irisin incubation or not. Then, apoptosis, viability, migration, NO secretion, and inflammasome activation were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The hypoxic CMECs exhibited increased apoptosis, impaired viability, and migration, even decreased NO secretion and enhanced inflammasome activation. Moreover, irisin incubation decreased NLRP3 activation and attenuated cell injury in HG\/HF cultured CMECs subjected to H\/R injury, which was abolished by NLRP3 inflammasome activation. Meanwhile, NLRP3 inflammasome siRNA also attenuated H\/R injury in CMECs under HG\/HF condition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The current study demonstrated for the first time that irisin inhibits NLRP3 inflammasome activation in CMECs as a novel mechanism in myocardial ischemia\/reperfusion injury in diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12786","type":"journal-article","created":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T11:13:26Z","timestamp":1664018006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Irisin inhibits <scp>NLRP3<\/scp> inflammasome activation in <scp>HG<\/scp>\/<scp>HF<\/scp> incubated cardiac microvascular endothelial cells with H\/R injury"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9693-6422","authenticated-orcid":false,"given":"Chao","family":"Xin","sequence":"first","affiliation":[{"name":"PLA Rocket Force Characteristic Medical Center  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Xijing Hospital The Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningbo","family":"Hao","sequence":"additional","affiliation":[{"name":"PLA Rocket Force Characteristic Medical Center  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianan","family":"Wang","sequence":"additional","affiliation":[{"name":"PLA Rocket Force Characteristic Medical Center  Beijing 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