{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T10:07:11Z","timestamp":1768730831403,"version":"3.49.0"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002280","name":"Plastic Surgery Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The success of engineered tissues continues to be limited by time to vascularization and perfusion. Recently, we described a simple microsurgical approach, termed micropuncture (MP), which could be used to rapidly vascularize an adjacently placed scaffold from the recipient macrovasculature. Here we studied the long\u2010term persistence of the MP\u2010induced microvasculature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Segmental 60\u2009\u03bcm diameter MPs were created in the recipient rat femoral artery and vein followed by coverage with a simple Type 1 collagen scaffold. The recipient vasculature and scaffold were then wrapped <jats:italic>en bloc<\/jats:italic> with a silicone sheet to isolate intrinsic vascularization. Scaffolds were harvested at 28\u2009days post\u2010implantation for detailed analysis, including using a novel artificial intelligence (AI) approach.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MP scaffolds demonstrated a sustained increase of vascular density compared to internal non\u2010MP control scaffolds (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) secondary to increases in both vessel diameters (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and branch counts (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). MP scaffolds also demonstrated statistically significant increases in red blood cell (RBC) perfused lumens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study further highlights that the intrinsic MP\u2010induced vasculature continues to persist long\u2010term. Its combination of rapid and stable angiogenesis represents a novel surgical platform for engineered scaffold and graft perfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12835","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T15:49:00Z","timestamp":1699631340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Vascular persistence following precision micropuncture"],"prefix":"10.1111","volume":"31","author":[{"given":"Summer N.","family":"Horchler","sequence":"first","affiliation":[{"name":"Irvin S. Zubar Plastic Surgery Research Laboratory Penn State College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick C.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Irvin S. 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