{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:14:19Z","timestamp":1762254859039,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The endothelium regulates crucial aspects of vascular function, including hemostasis, vasomotor tone, proliferation, immune cell adhesion, and microvascular permeability. Endothelial cells (ECs), especially in arterioles, are pivotal for flow distribution and peripheral resistance regulation. Investigating vascular endothelium physiology, particularly in microvascular ECs, demands precise isolation and culturing techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Freshly isolated ECs are vital for examining protein expression, ion channel behavior, and calcium dynamics. Establishing primary endothelial cell cultures is crucial for unraveling vascular functions and understanding intact microvessel endothelium roles. Despite the significance, detailed protocols and comparisons with intact vessels are scarce in microvascular research. We developed a reproducible method to isolate microvascular ECs, assessing substrate influence by cultivating cells on fibronectin and gelatin matrix gels. This comparative approach enhances our understanding of microvascular endothelial cell biology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Microvascular mesenteric ECs expressed key markers (VE\u2010cadherin and eNOS) in both matrix gels, confirming cell culture purity. Under uncoated conditions, ECs were undetected, whereas proteins linked to smooth muscle cells and fibroblasts were evident.<\/jats:p><jats:p>Examining endothelial cell (EC) physiological dynamics on distinct matrix substrates revealed comparable cell length, shape, and Ca<jats:sup>2+<\/jats:sup> elevations in both male and female ECs on gelatin and fibronectin matrix gels. Gelatin\u2010cultured ECs exhibited analogous membrane potential responses to acetylcholine (ACh) or adenosine triphosphate (ATP), contrasting with their fibronectin\u2010cultured counterparts. In the absence of stimulation, fibronectin\u2010cultured ECs displayed a more depolarized resting membrane potential than gelatin\u2010cultured ECs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Gelatin\u2010cultured ECs demonstrated electrical behaviors akin to intact endothelium from mouse mesenteric arteries, thus advancing our understanding of endothelial cell behavior within diverse microenvironments.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12859","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T11:30:50Z","timestamp":1717155050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Matrix Gel Variations on Primary Culture of Microvascular Endothelial Cell Function"],"prefix":"10.1111","volume":"31","author":[{"given":"P\u00eda\u00a0C.","family":"Burboa","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Physiology &amp; Neuroscience, New Jersey Medical School, Rutgers The State University of New Jersey  Newark New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana\u00a0C.","family":"Corr\u00eaa\u2010Velloso","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology &amp; Neuroscience, New Jersey Medical School, Rutgers The State University of New Jersey  Newark New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Arriagada","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Biol\u00f3gicas y Qu\u00edmicas, Facultad de Medicina y Ciencia Universidad San Sebasti\u00e1n  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew\u00a0P.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology &amp; Neuroscience, New Jersey Medical School, Rutgers The State University of New Jersey  Newark New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter\u00a0N.","family":"Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology &amp; Neuroscience, New Jersey Medical School, Rutgers The State University of New Jersey  Newark New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2823-6619","authenticated-orcid":false,"given":"Mauricio\u00a0A.","family":"Lillo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology &amp; Neuroscience, New Jersey Medical School, Rutgers The State University of New Jersey  Newark New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000255690.03436.ae"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000255691.76142.4a"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006429"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2019.03.008"},{"key":"e_1_2_11_6_1","first-page":"65","volume-title":"The Cardiovascular System\u2010Physiology, Diagnostics and Clinical Implications","author":"Lillo M. 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