{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:10:35Z","timestamp":1759777835307,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to clarify the effect of Type I diabetes (DIA) on transcapillary <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> gradients, which are oxygen\u2010driving factors between the blood and the interstitium, in the contracting muscle of rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wistar male rats were divided into the diabetic (streptozocin i.p.) and sham groups. Microvascular and interstitial <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> were measured in the extensor digitorum longus muscle during electrical stimulation\u2010induced muscle contraction, using the phosphorescence quenching method. Transcapillary <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> gradient, \u0394<jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub>, was calculated as microvascular minus interstitial <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Resting microvascular <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> was higher in the diabetic group than in the sham group (6.3\u2009\u00b1\u20091.7 vs. 4.7\u2009\u00b1\u20090.9\u2009mmHg, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) and remained for 180\u2009s. Interstitial <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> from rest to muscle contraction did not differ between the groups. The \u0394<jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> was higher in the diabetic group than in the sham group at rest and during muscle contraction (4.03\u2009\u00b1\u20091.42 vs. 2.46\u2009\u00b1\u20090.90\u2009mmHg at rest; 3.67\u2009\u00b1\u20091.51 vs. 2.22\u2009\u00b1\u20090.65\u2009mmHg during muscle contraction, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Marked muscle atrophy was observed in the diabetic group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DIA increased microvascular and transcapillary <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> gradients in the skeletal muscle. The enhanced <jats:italic>P<\/jats:italic>O<jats:sub>2<\/jats:sub> gradients were maintained from rest to muscle contraction in diabetic muscle.<\/jats:p><\/jats:sec>","DOI":"10.1111\/micc.12870","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T18:13:45Z","timestamp":1716920025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcapillary <scp><i>P<\/i>O<sub>2<\/sub><\/scp> Gradients in Contracting Muscles of Type I Diabetic Rats"],"prefix":"10.1111","volume":"31","author":[{"given":"Ren","family":"Takamizawa","sequence":"first","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8639-2521","authenticated-orcid":false,"given":"Kazuki","family":"Hotta","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Kitasato University School of Allied Health Sciences  Sagamihara Kanagawa Japan"},{"name":"Department of Rehabilitation Sciences Kitasato University Graduate School of Medical Sciences  Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Fujii","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Ikegami","sequence":"additional","affiliation":[{"name":"Department of Engineering Science University of Electro\u2010Communications  Chofu Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Hitosugi","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuro","family":"Inoue","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Tamiya","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuhiro","family":"Tsubaki","sequence":"additional","affiliation":[{"name":"Institute for Human Movement and Medical Sciences Niigata University of Health and Welfare  Niigata Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1007\/s00592-009-0114-7"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1210\/endrev\/bnaa025"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1152\/ajpendo.00267.2021"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1007\/s00125-017-4360-x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.4239\/wjd.v6.i17.1323"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1016\/j.diabres.2007.10.033"},{"issue":"4","key":"e_1_2_10_8_1","first-page":"H1502","article-title":"Microcirculatory Structure\u2010Function Relationships in Skeletal Muscle of Diabetic Rats","volume":"266","author":"Sexton W. 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