{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T09:45:25Z","timestamp":1772358325228,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL160606\u201001"],"award-info":[{"award-number":["HL160606\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5T32HL007736\u201325"],"award-info":[{"award-number":["5T32HL007736\u201325"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30CA118100"],"award-info":[{"award-number":["P30CA118100"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Endothelial cells (ECs) express an array of integral membrane proteins, including ion channels and transporters that contribute to blood flow regulation and cell\u2013cell communication. Many of these membrane proteins are regulated by plasma membrane cholesterol content. The ATP\u2010binding cassette family A1 (ABCA1) transporter is a regulator of membrane cholesterol content. We have shown increased ABCA1 mRNA expression and reduced EC membrane cholesterol in resistance mesenteric arteries compared to conduit arteries. Previous studies suggest shear stress (SS) can increase or decrease ABCA1 expression in a cell\u2010type\u2010dependent manner.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis<\/jats:title>\n                    <jats:p>\n                      SS sustains lower EC membrane cholesterol concentration through ABCA1\u2010mediated cholesterol transport, facilitating H\n                      <jats:sub>2<\/jats:sub>\n                      S\u2010mediated vasodilation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The effect of SS on ABCA1 and membrane cholesterol content was assessed in pressurized mesenteric arteries from male Sprague\u2013Dawley rats and cultured human aortic endothelial cells. Pressure myography was used to assess the effects of ABCA1 inhibition on H\n                      <jats:sub>2<\/jats:sub>\n                      S\u2010mediated vasodilation. Filipin was used to assess EC membrane cholesterol content.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      SS increased ABCA1 expression in the endothelium of mesenteric arteries and cultured human aortic endothelial cells and markedly reduced EC membrane cholesterol. Inhibition of ABCA1 increased EC membrane cholesterol content and abolished H\n                      <jats:sub>2<\/jats:sub>\n                      S\u2010induced vasodilation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SS facilitation of EC\u2010dependent vasodilation appears to be mediated by membrane cholesterol content.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70029","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T06:01:30Z","timestamp":1759730490000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Shear Stress Regulates\n                    <scp>ABCA1<\/scp>\n                    \u2010Dependent Membrane Cholesterol Content in Endothelial Cells Facilitating\n                    <scp>\n                      H\n                      <sub>2<\/sub>\n                      S\n                    <\/scp>\n                    \u2010Dependent Vasodilation"],"prefix":"10.1111","volume":"32","author":[{"given":"Jacob R.","family":"Anderson","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Science Center, MSC08 4750  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy L.","family":"Kanagy","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Science Center, MSC08 4750  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura V.","family":"Gonzalez Bosc","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Science Center, MSC08 4750  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay S.","family":"Naik","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology University of New Mexico Health Science Center, MSC08 4750  Albuquerque New Mexico USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.306940"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10333"},{"key":"e_1_2_9_4_1","volume-title":"Vascular Effects of Hydrogen Sulfide: Methods and Protocols","year":"2019"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.116.08964"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4645"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00329.2016"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.05.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11091680"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.02882"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00126.2022"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-3-3-281"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00005.2005"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.143487"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-022-00748-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.300818"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00861"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2011.0177"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00053.2021"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00465.2016"},{"key":"e_1_2_9_21_1","volume-title":"National Institutes of Health, Bethesda, Maryland, USA","author":"Rasband W. S.","year":"1997"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01021.2020"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213173"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12774"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-022-05802-z"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1352259"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1007-4376(08)60066-0"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2006.07.018"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M300418-JLR200"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113479"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14960"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00164-2"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.5812\/cardiovascmed.13892"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310695200"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.11.077"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.356261"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410987200"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.05.034"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200210135"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570161114666151202205139"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03392-y"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780193"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103264200"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407817200"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202996200"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2008.01261.x"}],"container-title":["Microcirculation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/micc.70029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T05:29:30Z","timestamp":1761802170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/micc.70029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":45,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/micc.70029"],"URL":"https:\/\/doi.org\/10.1111\/micc.70029","archive":["Portico"],"relation":{},"ISSN":["1073-9688","1549-8719"],"issn-type":[{"value":"1073-9688","type":"print"},{"value":"1549-8719","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70029"}}