{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:12:59Z","timestamp":1791313979669,"version":"4.3.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T00:00:00Z","timestamp":1767484800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Handheld vital microscopy (HVM) enables bedside visualization of the microcirculation, with major improvements from sidestream darkfield (SDF) to incident dark field (IDF) imaging. Although IDF offers high\u2010resolution images, standard analysis methods require down\u2010sampling to match SDF's lower field of view (FOV) and pixel density. MicroTools, an automated stabilization and analysis algorithm, has previously been validated for use with SDF image sequences. This study aimed to assess the accuracy and precision of microcirculatory analysis using MicroTools on full\u2010frame, high\u2010resolution IDF images.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Image sequences from a previous study were re\u2010analyzed in both IDF and down\u2010sampled SDF formats. Microcirculatory parameters\u2014including total vessel density (TVD), functional capillary density, and red blood cell velocity (RBCv)\u2014were compared between formats. MicroTools' new stabilization algorithm was also evaluated against existing algorithms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Full\u2010frame IDF analysis increased the FOV by 200% and pixel density by 66%, enhancing capillary detection and TVD measurements. RBCv values were lower with IDF images. The updated stabilization algorithm showed performance comparable to prior methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MicroTools analysis of full\u2010frame IDF images improved measurement accuracy and vessel detection, with excellent agreement to standard software. Its new stabilization algorithm proved equally robust, supporting broader adoption in clinical research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70045","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T17:38:50Z","timestamp":1767548330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Improved Detection of Capillaries in High\u2010Resolution Handheld Vital Microscopy by Use of the\n                    <scp>MicroTools<\/scp>\n                    Advanced Computer Vision Algorithm"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4664-1861","authenticated-orcid":false,"given":"Philippe","family":"Guerci","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine University Hospital of Nancy  Nancy France"},{"name":"INSERM U111 University of Lorraine  Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Ince","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Erasmus MC, University Medical Center  Rotterdam the Netherlands"},{"name":"Active Medical BV  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olcay","family":"Dilken","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Erasmus MC, University Medical Center  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibaut","family":"Belveyre","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Critical Care Magellan Medico\u2010Surgical Center, CHU Bordeaux  Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coline","family":"Lapoix","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine University Hospital of Nancy  Nancy France"},{"name":"INSERM U111 University of Lorraine  Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4028-9588","authenticated-orcid":false,"given":"Jonathan","family":"Montomoli","sequence":"additional","affiliation":[{"name":"Infermi Hospital Department of Anesthesia and Intensive Care  Rimini Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2765-881X","authenticated-orcid":false,"given":"Matthias P.","family":"Hilty","sequence":"additional","affiliation":[{"name":"University Hospital of Zurich Institute of Intensive Care Medicine  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/13529"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.15.015101"},{"key":"e_1_2_15_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-015-0040-7"},{"key":"e_1_2_15_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-018-5070-7"},{"key":"e_1_2_15_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000729"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-152013"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0473-8"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000004491"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-017-0139-0"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2013.06.015"},{"issue":"8","key":"e_1_2_15_12_1","first-page":"674","article-title":"An Iterative Image Registration Technique With an Application to Stereo Vision","volume":"8","author":"Lucas B. 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