{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T07:22:40Z","timestamp":1786864960193,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF 15OC0016674"],"award-info":[{"award-number":["NNF 15OC0016674"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020733","name":"Sino-Danish Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020733","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Acute ischemic stroke (AIS) is one of the leading causes of death and disabilities, and as such, it is of utmost importance to identify novel treatment options. Remote ischemic conditioning (RIC) is a promising non\u2010invasive treatment that is thought to activate the body's own protective mechanisms against damaging ischemia. Here, we study the transcriptomic impact of microRNAs (miRNAs) that are upregulated by RIC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using RNA sequencing, we investigated the transcriptional changes in human brain microvascular endothelial cells (HBMECs) transfected with four selected RIC\u2010upregulated miRNAs (RIC\u2010miRNAs), miR\u201016\u20105p, miR\u2010144\u20103p, miR\u2010182\u20105p, and miR\u2010451a, under oxygen and glucose deprivation (OGD) and reoxygenation\u2014mimicking the initial stages of AIS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Pronounced transcriptional changes were present after RIC\u2010miRNA transfection, with 149 unique downregulated and 212 upregulated differentially expressed genes in HBMECs after OGD and RIC\u2010miRNA transfection compared to all other conditions. These genes were involved in pathways of energy metabolism and cell cycle regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study suggests that the selected RIC\u2010miRNAs regulate pathways that may facilitate endothelial cell survival, recovery, and remodeling events from ischemic damage, adding to the knowledge of the pathways affected by RIC during stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70060","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:01:59Z","timestamp":1777701719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Insights Into Remote Ischemic Conditioning\n                    <scp>miRNA<\/scp>\n                    Effects on Brain Endothelial Cells During Ischemia\u2013Reperfusion"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2350-547X","authenticated-orcid":false,"given":"Katrine Tang","family":"Stenz","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University  Aarhus Denmark"},{"name":"Sino\u2010Danish College (SDC) University of Chinese Academy Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper","family":"Just","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University  Aarhus Denmark"},{"name":"Department of Molecular Medicine Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenghui","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Developmental Biology Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Ravn","family":"Lassen","sequence":"additional","affiliation":[{"name":"Department of Cardiology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Vissing","sequence":"additional","affiliation":[{"name":"Department of Public Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Developmental Biology Chinese Academy of Sciences  Beijing China"},{"name":"School of Future Technology University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2701-2741","authenticated-orcid":false,"given":"Kim Ryun","family":"Drasbek","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Center of Functionally Integrative Neuroscience Aarhus University  Aarhus Denmark"},{"name":"Sino\u2010Danish College (SDC) University of Chinese Academy Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/17474930211065917"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01064-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207609"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00279.2015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1411587"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804656"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199512143332401"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60584-5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1706442"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1804355"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2009.116"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(13)70100-5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000196957.55928.ab"},{"key":"e_1_2_10_15_1","first-page":"4","article-title":"Ischemia\u2010Reperfusion Injury in the Brain: Mechanisms and Potential Therapeutic Strategies","volume":"5","author":"Lin L.","year":"2016","journal-title":"Biochemistry & Pharmacology. 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