{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T10:14:57Z","timestamp":1779963297509,"version":"3.53.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL149762"],"award-info":[{"award-number":["R01HL149762"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R00HL151889"],"award-info":[{"award-number":["R00HL151889"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R56HL169223"],"award-info":[{"award-number":["R56HL169223"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R21AG085331"],"award-info":[{"award-number":["R21AG085331"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008899","name":"University of South Carolina","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008899","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Perivascular adipose tissue (PVAT) surrounds most peripheral blood vessels and exerts an anti\u2010contractile influence through paracrine mediators. Although numerous vasoactive factors have been identified, the mechanisms linking adipocyte metabolism to PVAT\u2010dependent modulation of vascular tone remain poorly defined. Because adipocytes store energy as triglycerides hydrolyzed by adipose triglyceride lipase (ATGL) and hormone\u2010sensitive lipase (HSL) to generate free fatty acids, we hypothesized that lipolysis\u2010derived fatty acids may contribute to PVAT's anti\u2010contractile actions through activation of long\u2010chain fatty acid\u2010sensing G protein\u2010coupled receptors, Gpr40 and\/or Gpr120.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mesenteric resistance arteries (MRAs) from adult Wistar rats were studied using wire myography, with or without PVAT, and pharmacological agonists\/antagonists and endothelial denudation were used to study signaling. To examine changes in hypertension, PVAT and MRAs from spontaneously hypertensive rats (SHRs) were analyzed by western blotting, and plasma from non\u2010fasting or fasting SHR was assessed by untargeted lipidomics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In Wistar rats, inhibition of ATGL, but not HSL, abolished PVAT's anti\u2010contractile effect, and blockade of Gpr40, but not Gpr120, similarly diminished this response, identifying ATGL and Gpr40 as important mediators. Activation of Gpr40 in PVAT\u2010 and endothelium\u2010denuded MRAs further recapitulated the anti\u2010contractile effect in a \u03b2\u2010arrestin\u2010dependent manner. In SHR, PVAT ATGL expression was significantly upregulated and MRA Gpr40 expression tended to increase. However, circulating Gpr40 ligand abundance was largely unchanged between strains, suggesting that impaired ligand availability is unlikely to underlie PVAT dysfunction in hypertension.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings identify a previously unrecognized ATGL\u2010Gpr40 signaling axis linking adipocyte triglyceride metabolism to PVAT\u2010mediated regulation of vascular tone.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70063","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T06:33:44Z","timestamp":1777876424000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adipose Triglyceride Lipase and Gpr40 Contribute to the Anti\u2010Contractile Effect of Perivascular Adipose Tissue"],"prefix":"10.1111","volume":"33","author":[{"given":"Miranda","family":"Hyatt","sequence":"first","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinaldo","family":"Rodrigues Dos Passos","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenix A.","family":"Araujo","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Gon\u00e7alo Moniz Institute Oswaldo Cruz Foundation  Salvador BA Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily W.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily W.","family":"Waigi","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0780-4290","authenticated-orcid":false,"given":"Tiago J.","family":"Costa","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dar\u00edzy F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Gon\u00e7alo Moniz Institute Oswaldo Cruz Foundation  Salvador BA Brazil"},{"name":"Department of Bioregulation, Health Science Institute Federal University of Bahia  Salvador BA Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9403-305X","authenticated-orcid":false,"given":"R. Clinton","family":"Webb","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Biomedical Engineering, Molinaroli College of Engineering and Computing University of South Carolina  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0815-3568","authenticated-orcid":false,"given":"Camilla F.","family":"Wenceslau","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Biomedical Engineering, Molinaroli College of Engineering and Computing University of South Carolina  Columbia South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron G.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Cardiovascular Translational Research Center University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine\u2010Columbia  Columbia South Carolina USA"},{"name":"Department of Biomedical Engineering, Molinaroli College of Engineering and Computing University of South Carolina  Columbia South Carolina 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