{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:04:58Z","timestamp":1791475498092,"version":"4.3.4"},"reference-count":75,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL168568"],"award-info":[{"award-number":["R01HL168568"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F31HL179791"],"award-info":[{"award-number":["F31HL179791"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Recent clinical studies have suggested that glucagon\u2010like peptide\u20101 receptor (GLP\u20101R) agonists may be effective therapies to treat or reduce the risk of developing secondary lymphedema. In this study, we aimed to (1) determine if GLP\u20101Rs are present in the lymphatic vasculature and characterize their expression, and (2) assess the effects of GLP\u20101R agonism on the contractile function of collecting lymphatic vessels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We assessed the expression of GLP\u20101Rs in and around the lymphatic vasculature by single\u2010cell RNA sequencing and fluorescence confocal microscopy, and evaluated the direct effects of GLP\u20101R agonist, semaglutide, on modulating lymphatic contractility using pressure myography.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Expression of\n                      <jats:italic>Glp1r<\/jats:italic>\n                      (encoding GLP\u20101Rs) was detected exclusively in lymphatic endothelial cells. Pharmacological activation of GLP\u20101Rs led to robust vasodilation and an increase in the pumping capacity of isolated collecting lymphatics from WT, diet\u2010induced obese, and hypercholesterolemic ApoE KO mice. The GLP\u20101R\u2010mediated response was in part facilitated by nitric oxide and its potential interaction with NaV channels; however, additional signaling remains to be elucidated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results revealed a direct, beneficial effect of GLP\u20101R agonism on lymphatic pumping capacity mediated by robust vasodilation, allowing lymphatics to accommodate and displace larger fluid volumes while maintaining strong and highly efficient contractions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70070","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:55:35Z","timestamp":1780466135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>GLP<\/scp>\n                    \u20101 Receptors Are Enriched in the Lymphatic Endothelium and Their Pharmacological Activation With Semaglutide Improves the Pumping Capacity of Lymphatic Vessels"],"prefix":"10.1111","volume":"33","author":[{"given":"Mary E.","family":"Schulz","sequence":"first","affiliation":[{"name":"Department of Pharmacology Tulane University School of Medicine  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria L.","family":"Akerstrom","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane University School of Medicine  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph H.","family":"Dayan","sequence":"additional","affiliation":[{"name":"The Institute for Advanced Reconstruction  Glen Rock New Jersey USA"},{"name":"The Institute for Lymphatic Surgery &amp; Innovation  Glen Rock New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Castorena\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Tulane University School of Medicine  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12609-023-00491-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23126621"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318307"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1413.014"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X9700200408"},{"key":"e_1_2_10_7_1","unstructured":"S. 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