{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:35:52Z","timestamp":1786545352485,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Zinc finger E\u2010box\u2010binding homeobox\u00a01 (ZEB1) is a transcription factor primarily known for its regulatory roles in epithelial\u2010to\u2010mesenchymal transition (EMT) and cell fate determination. Recent studies suggest that endothelial ZEB1 signaling promotes blood vessel growth and reduces junctional integrity, although the underlying mechanisms remain unclear. Notably, the role of ZEB1 in the lymphatic vasculature is unknown, and the regulation of lymphatic integrity by VE\u2010cadherin remains poorly defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here, using an integrated proteomic and transcriptomic approach, we identify ZEB1\u2010dependent signaling pathways associated with cell\u2013cell junction reorganization in lymphatic endothelial cells (LECs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Loss of ZEB1 reduced VE\u2010cadherin phosphorylation at pY731 and pY685 and was accompanied by decreased monolayer resistance and impedance, together with increased leukocyte transendothelial migration. ZEB1 knockdown also reduced YES tyrosine kinase expression and altered YAP1 expression and junctional localisation, changes that were associated with reduced VE\u2010cadherin phosphorylation. Silencing YAP1 in HDLECs similarly reduced VE\u2010cadherin phosphorylation and impaired barrier integrity, recapitulating aspects of the phenotype observed following ZEB1 knockdown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Collectively, these findings suggest that ZEB1 contributes to lymphatic endothelial barrier maintenance in association with altered YAP1 and YES signaling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70076","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T16:18:11Z","timestamp":1782404291000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ZEB1<\/scp>\n                    Modifies\n                    <scp>VE<\/scp>\n                    \u2010Cadherin Signaling in Lymphatic Endothelial Cells"],"prefix":"10.1111","volume":"33","author":[{"given":"Nada S.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Endothelial Quiescence Group, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"},{"name":"Faculty of Pharmacy Ain Shams University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph L.","family":"Horder","sequence":"additional","affiliation":[{"name":"Endothelial Quiescence Group, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles T.","family":"Cresswell","sequence":"additional","affiliation":[{"name":"Endothelial Quiescence Group, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poppy 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Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Hallwood","sequence":"additional","affiliation":[{"name":"Tumour Vascular Biology Laboratories, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Tumour Vascular Biology Laboratories, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0821-4575","authenticated-orcid":false,"given":"Michael A.","family":"Portelli","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Research, National Institute for Health Research Nottingham Biomedical Research Centre, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Fezovich","sequence":"additional","affiliation":[{"name":"Hypoxia and Acidosis Group, Centre for Cancer Sciences, School of Medicine, Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Spanos","sequence":"additional","affiliation":[{"name":"Wellcome Discovery Research Platform for Hidden Cell Biology University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6490-2412","authenticated-orcid":false,"given":"David S.","family":"Gardner","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Science University of Nottingham  Nottingham 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