{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:07:09Z","timestamp":1788934029834,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Endothelial and microvascular dysfunction are key features of Long COVID. Disturbances in cellular redox balance, reflected by altered nicotinamide adenine dinucleotide (NAD\n                      <jats:sup>+<\/jats:sup>\n                      \/NADH) dynamics, may underlie vascular impairment. Flow\u2010Mediated Skin Fluorescence (FMSF) evaluates microvascular function by monitoring NADH fluorescence during ischemia and reperfusion. We integrated FMSF\u2010derived microvascular phenotyping with targeted NAD\n                      <jats:sup>+<\/jats:sup>\n                      metabolite profiling to determine whether altered NAD\n                      <jats:sup>+<\/jats:sup>\n                      metabolism is associated with impaired microvascular responses in Long COVID.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Microvascular function was assessed in 36 patients with Long COVID and 47 age\u2010matched controls using FMSF. NADH fluorescence changes during ischemia and hyperemia were analyzed as markers of endothelial responsiveness. NAD\n                      <jats:sup>+<\/jats:sup>\n                      and related metabolites were measured using high\u2010performance liquid chromatography and mass spectrometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Patients with Long COVID showed impaired FMSF parameters, including blunted ischemic responses and delayed recovery after hyperemia, indicating microvascular dysfunction. These changes were accompanied by a reduced NAD\n                      <jats:sup>+<\/jats:sup>\n                      \/NADH ratio and lower NADP levels, consistent with redox imbalance. Abnormal fluorescence profiles were associated with altered NAD\n                      <jats:sup>+<\/jats:sup>\n                      metabolism, including reduced precursor availability and accumulation of degradation products. Higher NR concentrations showed associations with selected microvascular and eNOS\u2010related parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      FMSF provides a clinically applicable tool for detecting microvascular dysfunction in Long COVID. NAD\n                      <jats:sup>+<\/jats:sup>\n                      redox imbalance is linked to impaired microcirculatory responses, supporting FMSF as a functional marker of microvascular impairment associated with altered NAD\n                      <jats:sup>+<\/jats:sup>\n                      metabolism.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/micc.70085","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:46:46Z","timestamp":1788932806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microvascular Dysfunction and Redox Imbalance in Long\n                    <scp>COVID<\/scp>"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3017-3869","authenticated-orcid":false,"given":"Adela","family":"Lewandowska","sequence":"first","affiliation":[{"name":"Department of Biochemistry Medical University of Gdansk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6819-326X","authenticated-orcid":false,"given":"Dominika","family":"Sawicka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical University of Gdansk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5727-2402","authenticated-orcid":false,"given":"Aleksandra","family":"J\u00f3\u017awiak","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical University of Gdansk  Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8219-7497","authenticated-orcid":false,"given":"Agata","family":"J\u0119drzejewska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Medical University of Gdansk  Gda\u0144sk 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