{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:53:57Z","timestamp":1791478437178,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010398","name":"Metro South Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n Explore potential associations between sublingual microcirculatory measures and plasma plasminogen activator inhibitor\u2010one concentrations in patients attending the kidney transplant assessment clinic and healthy controls.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A single\u2010center cohort study was performed by retrospectively recruiting 44 kidney transplant assessment clinic attendees and 15 nested non\u2010renal failure controls who were selected from a larger pool (\n N<\/jats:italic>\n \u2009=\u200944). The nested controls were frequency matched to the kidney transplant assessment clinic cohort by sex, ethnicity and diabetes. Sidestream dark field videomicroscopy (MicroScan, Microvision Medical, Amsterdam) of the sublingual microcirculation allowed quantification of five microcirculatory parameters in all patients: total vessel density, microvascular flow index, portion of perfused vessels, heterogeneity index and perfused vessel density. Plasma plasminogen activator inhibitor\u2010one concentrations were determined using sandwich ELISA. Median (IQR) were reported for non\u2010normally distributed data. Mann\u2013Whitney\u2010Wilcoxon tests were performed to compare the distribution of ranks between non\u2010normally distributed groups (\n U<\/jats:italic>\n \u2010statistic) with\n p<\/jats:italic>\n \u2009<\u20090.05 considered statistically significant. The Benjamini\u2013Hochberg adjustment was applied to nominal\n p<\/jats:italic>\n \u2010values (\n q<\/jats:italic>\n \u2010value) to account for inflation of the false discovery rate across multiple exploratory analyses, with\n q<\/jats:italic>\n \u2009<\u20090.05 considered statistically significant.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Plasminogen activator inhibitor\u2010one levels were significantly higher in controls than those attending the transplant clinic (18.0 (11.5), 10.2 (6.8) ng\/mL; U\u2009=\u2009158,\n p<\/jats:italic>\n \u2009=\u20090.006). Among kidney transplant patients, nominal associations revealed PAI\u20101 was inversely correlated with hemodynamic parameters perfused vessel density (\n p<\/jats:italic>\n \u2009=\u20090.04,\n r<\/jats:italic>\n \u2009=\u2009\u22120.32) and microvascular flow index (\n p<\/jats:italic>\n \u2009=\u20090.01,\n r<\/jats:italic>\n \u2009=\u2009\u22120.38); neither survived the Benjamini\u2013Hochberg adjustment (\n q<\/jats:italic>\n \u2009=\u20090.24 and\n q<\/jats:italic>\n \u2009=\u20090.12, respectively). No other correlations between PAI\u20101 and any of the sublingual microcirculatory measures survived the Benjamini\u2013Hochberg adjustment in both cohorts.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n PAI\u20101 is potentially associated with maladaptive hemodynamic changes in the sublingual microcirculation, implying its dysregulation may potentiate microvascular dysfunction. The results, however, must be interpreted with caution as no associations survived the Benjamini\u2013Hochberg correction.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/micc.70089","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:09:52Z","timestamp":1791475792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Association Between Sublingual Microvascular Dysfunction and Plasminogen Activator Inhibitor\u2010One Levels in Chronic Kidney Disease Patients: An Exploratory Study"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7036-2187","authenticated-orcid":false,"given":"Nikhil","family":"Kumar","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health, Behavioural Science and Medicine The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1978-782X","authenticated-orcid":false,"given":"Ryan","family":"Homes","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health, Behavioural Science and Medicine The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1539-4167","authenticated-orcid":false,"given":"Halley","family":"Ravuri","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health, Behavioural Science and Medicine The University of Queensland Brisbane Queensland Australia"},{"name":"Centre for Cardiovascular Health and Research University of Queensland Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2115-6306","authenticated-orcid":false,"given":"Mark","family":"Midwinter","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health, Behavioural Science and Medicine The University of Queensland Brisbane Queensland Australia"},{"name":"Centre for Cardiovascular Health and Research University of Queensland Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"issue":"5","key":"e_1_2_14_2_1","doi-asserted-by":"crossref","DOI":"10.1111\/micc.12819","article-title":"The Sublingual Microcirculation and Frailty Index in Chronic Kidney Disease Patients","volume":"30","author":"Homes R.","year":"2023","journal-title":"Microcirculation"},{"issue":"4","key":"e_1_2_14_3_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3109\/03009730903225537","article-title":"Serum ADMA Concentration \u2013 An Independent Factor Determining FMD Impairment in Cardiac Syndrome X","volume":"114","author":"Haberka M.","year":"2009","journal-title":"Upsala Journal of Medical Sciences"},{"key":"e_1_2_14_4_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.archger.2016.10.007","article-title":"Frailty, and Chronic Kidney Disease: A Systematic Review","volume":"68","author":"Chowdhury R.","year":"2017","journal-title":"Archives of Gerontology and Geriatrics"},{"issue":"2","key":"e_1_2_14_5_1","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/ckj\/sfx134","article-title":"Frailty, and Chronic Kidney Disease: Current Evidence and Continuing Uncertainties","volume":"11","author":"Nixon A. 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