{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:23:18Z","timestamp":1791066198340,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,11,19]],"date-time":"2012-11-19T00:00:00Z","timestamp":1353283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"Summary<\/jats:title>The diffusible factor synthase XanB2<\/jats:roman><\/jats:styled-content>, originally identified in X<\/jats:styled-content>anthomonas campestris<\/jats:italic> pv. campestris<\/jats:italic> (Xcc<\/jats:italic><\/jats:styled-content>), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2<\/jats:roman><\/jats:styled-content> is a unique bifunctional chorismatase that hydrolyses chorismate, the end\u2010product of the shikimate pathway, to produce 3\u2010hydroxybenzoic acid (3\u2010HBA<\/jats:styled-content>) and 4\u2010HBA<\/jats:styled-content>. 3\u2010HBA<\/jats:styled-content> and 4\u2010HBA<\/jats:styled-content> are respectively associated with the yellow pigment xanthomonadin biosynthesis and antioxidant activity in Xcc<\/jats:italic><\/jats:styled-content>. We further demonstrate that XanB2<\/jats:roman><\/jats:styled-content> is a structurally novel enzyme with three putative domains. It catalyses 3\u2010HBA<\/jats:styled-content> and 4\u2010HBA<\/jats:styled-content> biosynthesis via a unique mechanism with the C<\/jats:styled-content>\u2010terminal YjgF<\/jats:styled-content>\u2010like domain conferring activity for 3\u2010HBA<\/jats:styled-content> biosynthesis and the N<\/jats:styled-content>\u2010terminal FGFG<\/jats:styled-content> motif\u2010containing domain responsible for 4\u2010HBA<\/jats:styled-content> biosynthesis. Furthermore, we show that Xcc<\/jats:italic><\/jats:styled-content> produces coenzyme Q<\/jats:styled-content>8 (CoQ<\/jats:styled-content>8) via a new biosynthetic pathway independent of the key chorismate\u2010pyruvate lyase UbiC<\/jats:styled-content>. XanB2<\/jats:roman><\/jats:styled-content> is the alternative source of 4\u2010HBA<\/jats:styled-content> for CoQ<\/jats:styled-content>8 biosynthesis. The similar CoQ<\/jats:styled-content>8 biosynthetic pathway, xanthomonadin biosynthetic gene cluster and XanB2<\/jats:roman><\/jats:styled-content> homologues are well conserved in the bacterial species within X<\/jats:styled-content>anthomonas<\/jats:italic>, X<\/jats:styled-content>ylella<\/jats:italic>, X<\/jats:styled-content>ylophilus<\/jats:italic>, P<\/jats:styled-content>seudoxanthomonas<\/jats:italic>, R<\/jats:styled-content>hodanobacter<\/jats:italic>, F<\/jats:styled-content>rateuria<\/jats:italic>, H<\/jats:styled-content>erminiimonas<\/jats:italic> and V<\/jats:styled-content>ariovorax<\/jats:italic>, suggesting that XanB2<\/jats:roman><\/jats:styled-content> may be a conserved metabolic link between the shikimate pathway, ubiquinone and xanthomonadin biosynthetic pathways in diverse bacteria.<\/jats:p>","DOI":"10.1111\/mmi.12084","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T05:11:17Z","timestamp":1351746677000},"page":"80-93","source":"Crossref","is-referenced-by-count":94,"title":["The diffusible factor synthase XanB2<\/scp><\/scp> is a bifunctional chorismatase that links the shikimate pathway to ubiquinone and xanthomonadins biosynthetic pathways"],"prefix":"10.1111","volume":"87","author":[{"given":"Lian","family":"Zhou","sequence":"first","affiliation":[{"name":"National Center for Molecular Characterization of GMOs and State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Molecular Characterization of GMOs and State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhe","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Natural Resources and Environment South China Agricultural University Guangzhou 510650 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Poplawsky","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil & Entomological Sciences University of Idaho Moscow ID 83844\u20102339 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangjun","family":"Lin","sequence":"additional","affiliation":[{"name":"National Center for Molecular Characterization of GMOs and State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bangshang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Instrumental Analysis Center Shanghai Jiao Tong University Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqing","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Natural Resources and Environment South China Agricultural University Guangzhou 510650 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tielin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Natural Resources and Environment South China Agricultural University Guangzhou 510650 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian\u2010Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Natural Resources and Environment South China Agricultural University Guangzhou 510650 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Wen","family":"He","sequence":"additional","affiliation":[{"name":"National Center for Molecular Characterization of GMOs and State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015773108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)92565-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.02.147"},{"key":"e_1_2_8_5_1","first-page":"209","article-title":"Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv. phaseolicola","volume":"9","author":"Bestwick C.S.","year":"1997","journal-title":"Plant Cell"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02837.x"},{"key":"e_1_2_8_7_1","volume-title":"Xylophilus ampelinus. 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