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We found the <jats:styled-content style=\"fixed-case\"><jats:roman>TbLOK1<\/jats:roman><\/jats:styled-content> protein located in discrete patches in the mitochondrial outer membrane. Knock\u2010down of <jats:styled-content style=\"fixed-case\"><jats:roman>TbLOK1<\/jats:roman><\/jats:styled-content> in procyclic trypanosomes caused the highly interconnected mitochondrial structure to collapse, forming an unbranched tubule remarkably similar to the streamlined organelle seen in the bloodstream form. Following <jats:styled-content style=\"fixed-case\">RNAi<\/jats:styled-content>, defects in mitochondrial respiration, inner membrane potential and mitochondrial transcription were observed. At later times following <jats:styled-content style=\"fixed-case\"><jats:roman>TbLOK1<\/jats:roman><\/jats:styled-content> depletion, <jats:styled-content style=\"fixed-case\">kDNA<\/jats:styled-content> was lost and a more drastic alteration in mitochondrial structure was found. Our results demonstrate the close relationship between organelle structure and function in trypanosomes.<\/jats:p>","DOI":"10.1111\/mmi.12089","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T02:36:50Z","timestamp":1358822210000},"page":"713-729","source":"Crossref","is-referenced-by-count":25,"title":["Mitochondrial shape and function in trypanosomes requires the outer membrane protein, <scp><scp>TbLOK1<\/scp><\/scp>"],"prefix":"10.1111","volume":"87","author":[{"given":"Megan L.","family":"Povelones","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry The Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calvin","family":"Tiengwe","sequence":"additional","affiliation":[{"name":"Department of Cell Biology The Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Gluenz","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology Oxford University  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Gull","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology Oxford University  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Englund","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry The Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Cell Biology The Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2011_SI1_13"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2007.09.001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.04.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.3.545"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E03-04-0225"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.1.137-151.2002"},{"key":"e_1_2_6_8_1","first-page":"289","article-title":"Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi\u2010defined medium. 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