{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:48:13Z","timestamp":1791247693477,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,12,19]],"date-time":"2012-12-19T00:00:00Z","timestamp":1355875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Summary<\/jats:title>OmpR<\/jats:roman><\/jats:styled-content> is a multifunctional DNA<\/jats:styled-content> binding regulator with orthologues in many enteric bacteria that exhibits classical regulator activity as well as nucleoid\u2010associated protein\u2010like characteristics. In the enteric pathogen S<\/jats:styled-content>almonella enterica<\/jats:italic>, using chromatin immunoprecipitation of OmpR<\/jats:roman><\/jats:styled-content>:FLAG<\/jats:styled-content> and nucleotide sequencing, 43 putative OmpR<\/jats:roman><\/jats:styled-content> binding sites were identified in S<\/jats:styled-content>.\u2009enterica<\/jats:italic> serovar T<\/jats:styled-content>yphi, 22 of which were associated with OmpR<\/jats:roman><\/jats:styled-content>\u2010regulated genes. Mutation of a sequence motif (TGTWACAW<\/jats:styled-content>) that was associated with the putative OmpR<\/jats:roman><\/jats:styled-content> binding sites abrogated binding of OmpR<\/jats:roman><\/jats:styled-content>:6\u00d7His to the tviA<\/jats:italic><\/jats:styled-content> upstream region. A core set of 31 orthologous genes were found to exhibit OmpR<\/jats:roman><\/jats:styled-content>\u2010dependent expression in both S<\/jats:styled-content>.<\/jats:italic> T<\/jats:styled-content>yphi and S<\/jats:styled-content>.<\/jats:italic> T<\/jats:styled-content>yphimurium. S<\/jats:styled-content>.<\/jats:italic> T<\/jats:styled-content>yphimurium\u2010encoded orthologues of two divergently transcribed OmpR<\/jats:roman><\/jats:styled-content>\u2010regulated operons (SL<\/jats:styled-content>1068\u201371 and SL<\/jats:styled-content>1066\u201367) had a putative OmpR<\/jats:roman><\/jats:styled-content> binding site in the inter\u2010operon region in S<\/jats:styled-content>.<\/jats:italic> T<\/jats:styled-content>yphi, and were characterized using in vitro<\/jats:italic> and in vivo<\/jats:italic> assays. These operons are widely distributed within S<\/jats:styled-content>.\u2009enterica<\/jats:italic> but absent from the closely related E<\/jats:styled-content>scherichia coli<\/jats:italic>. SL<\/jats:styled-content>1066 and SL<\/jats:styled-content>1067 were required for growth on N<\/jats:styled-content><\/jats:italic>\u2010acetylmuramic acid as a sole carbon source. SL<\/jats:styled-content>1068\u201371 exhibited sequence similarity to sialic acid uptake systems and contributed to colonization of the ileum and caecum in the streptomycin\u2010pretreated mouse model of colitis.<\/jats:p>","DOI":"10.1111\/mmi.12111","type":"journal-article","created":{"date-parts":[[2012,11,28]],"date-time":"2012-11-28T23:55:08Z","timestamp":1354146908000},"page":"526-538","source":"Crossref","is-referenced-by-count":68,"title":["ChIP<\/scp>\u2010seq and transcriptome analysis of the OmpR<\/scp><\/scp> regulon of S<\/scp>almonella enterica<\/i> serovars T<\/scp>yphi and T<\/scp>yphimurium reveals accessory genes implicated in host colonization"],"prefix":"10.1111","volume":"87","author":[{"given":"Timothy T.","family":"Perkins","sequence":"first","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Davies","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J.","family":"Klemm","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Rowley","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of East Anglia Norwich NR4\u20097TJ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Wileman","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"James","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Keane","sequence":"additional","affiliation":[{"name":"The Wellcome Trust Sanger Institute The Wellcome Trust Genome Campus, Hinxton Cambridge CB10\u20091SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan","family":"Maskell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Cambridge Madingley Road Cambridge CB3\u20090ES UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay C. 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