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Subsequent tissue colonization is mediated by interactions between <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content> surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen\u2010like protein\u20101, <jats:styled-content style=\"fixed-case\"><jats:roman>Scl1<\/jats:roman><\/jats:styled-content>, selectively binds the cellular form of fibronectin (<jats:styled-content style=\"fixed-case\">cFn<\/jats:styled-content>) and also contributes to <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content> biofilm formation on abiotic surfaces. One structural feature of <jats:styled-content style=\"fixed-case\">cFn<\/jats:styled-content>, which is predominantly expressed in response to tissue injury, is the presence of a spliced variant containing extra domain <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">EDA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">EIIIA<\/jats:styled-content>). We now report that <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content> biofilm formation is mediated by the <jats:styled-content style=\"fixed-case\"><jats:roman>Scl1<\/jats:roman><\/jats:styled-content> interaction with <jats:styled-content style=\"fixed-case\">EDA<\/jats:styled-content>\u2010containing <jats:styled-content style=\"fixed-case\">cFn<\/jats:styled-content>. Recombinant <jats:styled-content style=\"fixed-case\"><jats:roman>Scl1<\/jats:roman><\/jats:styled-content> proteins that bound <jats:styled-content style=\"fixed-case\">cFn<\/jats:styled-content> also bound recombinant <jats:styled-content style=\"fixed-case\">EDA<\/jats:styled-content> within the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2019 loop region recognized by the \u03b1<jats:sub>9<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrin. The extracellular 2\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> matrix derived from human dermal fibroblasts supports <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content> adherence and biofilm formation. Altogether, this work identifies and characterizes a novel molecular mechanism by which <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content> utilizes <jats:styled-content style=\"fixed-case\"><jats:roman>Scl1<\/jats:roman><\/jats:styled-content> to specifically target an extracellular matrix component that is predominantly expressed at the site of injury in order to secure host tissue colonization.<\/jats:p>","DOI":"10.1111\/mmi.12125","type":"journal-article","created":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T10:30:09Z","timestamp":1355135409000},"page":"672-689","source":"Crossref","is-referenced-by-count":40,"title":["The group <scp>A<\/scp> streptococcal collagen\u2010like protein\u20101, <scp><scp>Scl1<\/scp><\/scp>, mediates biofilm formation by targeting the extra domain <scp>A<\/scp>\u2010containing variant of cellular fibronectin expressed in wounded 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