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The <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>occidia are obligate intracellular pathogens that establish infection in their mammalian host via the enteric route. These parasites lack a mitochondrial pyruvate dehydrogenase complex but have preserved the degradation of branched\u2010chain amino acids (<jats:styled-content style=\"fixed-case\">BCAA<\/jats:styled-content>) as a possible pathway to generate acetyl\u2010<jats:styled-content style=\"fixed-case\">CoA<\/jats:styled-content>. Importantly, degradation of leucine, isoleucine and valine could lead to concomitant accumulation of propionyl\u2010<jats:styled-content style=\"fixed-case\">CoA<\/jats:styled-content>, a toxic metabolite that inhibits cell growth. Like fungi and bacteria, the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>occidia possess the complete set of enzymes necessary to metabolize and detoxify propionate by oxidation to pyruvate via the 2\u2010methylcitrate cycle (2\u2010<jats:styled-content style=\"fixed-case\">MCC<\/jats:styled-content>). Phylogenetic analysis provides evidence that the 2\u2010<jats:styled-content style=\"fixed-case\">MCC<\/jats:styled-content> was acquired via horizontal gene transfer. In <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u2009gondii<\/jats:italic> tachyzoites, this pathway is split between the cytosol and the mitochondrion. Although the rate\u2010limiting enzyme 2\u2010methylisocitrate lyase is dispensable for parasite survival, its substrates accumulate in parasites deficient in the enzyme and its absence confers increased sensitivity to propionic acid. <jats:styled-content style=\"fixed-case\">BCAA<\/jats:styled-content> is also dispensable in tachyzoites, leaving unresolved the source of mitochondrial acetyl\u2010<jats:styled-content style=\"fixed-case\">CoA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/mmi.12139","type":"journal-article","created":{"date-parts":[[2012,12,25]],"date-time":"2012-12-25T09:13:44Z","timestamp":1356426824000},"page":"894-908","source":"Crossref","is-referenced-by-count":44,"title":["The 2\u2010methylcitrate cycle is implicated in the detoxification of propionate in <i><scp>T<\/scp>oxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"87","author":[{"given":"Julien","family":"Limenitakis","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular 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