{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:48:06Z","timestamp":1790930886603,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T00:00:00Z","timestamp":1364256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella<\/jats:italic> is the causative agent of the zoonotic disease brucellosis, which is endemic in many parts of the world. The success of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella<\/jats:italic> as pathogen relies in its ability to adapt to the harsh environmental conditions found in mammalian hosts. One of its main adaptations is the induction of the expression of different genes involved in respiration at low oxygen tension. In this report we describe a regulatory network involved in this adaptation. We show that <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella abortus<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">PrrBA<\/jats:styled-content> is a functional two\u2010component signal transduction system that responds to the redox status and acts as a global regulator controlling the expression of the regulatory proteins <jats:styled-content style=\"fixed-case\">NtrY<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">FnrN<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NnrA<\/jats:styled-content>, which are involved in the adaptation to survive at low oxygen tension. We also show that the two\u2010component systems <jats:styled-content style=\"fixed-case\">PrrBA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NtrYX<\/jats:styled-content> co\u2010ordinately regulate the expression of denitrification and high\u2010affinity cytochrome oxidase genes. Strikingly, a double mutant strain in the <jats:styled-content style=\"fixed-case\"><jats:italic>prrB<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>ntrY<\/jats:italic><\/jats:styled-content> genes is severely impaired in growth and virulence, while the <jats:styled-content style=\"fixed-case\"><jats:italic>ntrY<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>prrB<\/jats:italic><\/jats:styled-content> single mutant strains are similar to wild\u2010type <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009abortus<\/jats:italic>. The proposed regulatory network may contribute to understand the mechanisms used by <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella<\/jats:italic> for a successful adaptation to its replicative niche inside mammalian cells.<\/jats:p>","DOI":"10.1111\/mmi.12181","type":"journal-article","created":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T06:46:41Z","timestamp":1364280401000},"page":"222-233","source":"Crossref","is-referenced-by-count":36,"title":["The two\u2010component systems <scp>PrrBA<\/scp> and <scp>NtrYX<\/scp> co\u2010ordinately regulate the adaptation of <i><scp>B<\/scp>rucella abortus<\/i> to an oxygen\u2010limited environment"],"prefix":"10.1111","volume":"88","author":[{"given":"Mariela del Carmen","family":"Carrica","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda y Microbiolog\u00eda Molecular Fundaci\u00f3n Instituto Leloir (IIBBA\u2010CONICET)  Av. Patricias Argentinas 435 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda y Microbiolog\u00eda Molecular Fundaci\u00f3n Instituto Leloir (IIBBA\u2010CONICET)  Av. Patricias Argentinas 435 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Sieira","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica y Bioqu\u00edmica de Rhizobacterias Fundaci\u00f3n Instituto Leloir (IIBBA\u2010CONICET)  Av. Patricias Argentinas 435 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gast\u00f3n","family":"Paris","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda y Microbiolog\u00eda Molecular Fundaci\u00f3n Instituto Leloir (IIBBA\u2010CONICET)  Av. Patricias Argentinas 435 Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Alberto","family":"Goldbaum","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda y Microbiolog\u00eda Molecular Fundaci\u00f3n Instituto Leloir (IIBBA\u2010CONICET)  Av. 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