{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T22:49:30Z","timestamp":1783032570340,"version":"3.54.6"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T00:00:00Z","timestamp":1365379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>rypanosoma brucei<\/jats:italic> is the causative agent of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>uman <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>frican <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>rypanosomiasis. Trypanosomes are early diverged protozoan parasites and show significant differences in their gene expression compared with higher eukaryotes. Due to a lack of individual gene promoters, large polycistronic transcripts are produced and individual <jats:styled-content style=\"fixed-case\">mRNAs<\/jats:styled-content> mature by <jats:italic>trans<\/jats:italic>\u2010splicing and polyadenylation. In the absence of transcriptional control, regulation of gene expression occurs post\u2010transcriptionally mainly by control of transcript stability and translation. Regulation of <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> export from the nucleus to the cytoplasm might be an additional post\u2010transcriptional event involved in gene regulation. However, our knowledge about <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> export in trypanosomes is very limited. Although export factors of higher eukaryotes are reported to be conserved, only a few orthologues can be readily identified in the genome of <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u2009brucei<\/jats:italic>. Hence, biochemical approaches are needed to identify the export machinery of trypanosomes. Here, we report the functional characterization of the essential <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> export factor <jats:styled-content style=\"fixed-case\">TbMex<\/jats:styled-content>67. <jats:styled-content style=\"fixed-case\">TbMex<\/jats:styled-content>67 contains a unique and essential <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal zinc finger motif. Furthermore, we could identify two interacting export factors namely <jats:styled-content style=\"fixed-case\">TbMtr<\/jats:styled-content>2 and the karyopherin <jats:styled-content style=\"fixed-case\">TbIMP<\/jats:styled-content>1. Our data show that the general heterodimeric export receptor <jats:styled-content style=\"fixed-case\">Mex<\/jats:styled-content>67\u2010<jats:styled-content style=\"fixed-case\">Mtr<\/jats:styled-content>2 is conserved throughout the eukaryotic kingdom albeit exhibiting parasite\u2010specific features.<\/jats:p>","DOI":"10.1111\/mmi.12217","type":"journal-article","created":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T09:10:50Z","timestamp":1365412250000},"page":"728-739","source":"Crossref","is-referenced-by-count":49,"title":["The nuclear <scp>mRNA<\/scp> export receptor <scp>Mex<\/scp>67\u2010<scp>Mtr<\/scp>2 of <i><scp>T<\/scp>rypanosoma brucei<\/i> contains a unique and essential zinc finger motif"],"prefix":"10.1111","volume":"88","author":[{"given":"Anna","family":"Dostalova","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Science Charles University of Prague  Czech Republic"},{"name":"Institute of Cell Biology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro","family":"K\u00e4ser","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology University of Bern  Bern Switzerland"},{"name":"Department of Chemistry and Biochemistry University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Cristodero","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Schimanski","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000565"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.03.008"},{"key":"e_1_2_6_4_1","first-page":"289","article-title":"Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi\u2010defined medium. 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