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The genes designated <jats:styled-content style=\"fixed-case\">BAB<\/jats:styled-content>2_0837\u20130840 in the <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella abortus<\/jats:italic> 2308 genome sequence are predicted to encode a <jats:styled-content style=\"fixed-case\">CupII<\/jats:styled-content>\u2010type ferrous iron transporter homologous to the <jats:styled-content style=\"fixed-case\">FtrABCD<\/jats:styled-content> transporter recently described in <jats:italic>Bordetella<\/jats:italic>. To study the role of the <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">FtrABCD<\/jats:styled-content> in iron transport, an isogenic <jats:styled-content style=\"fixed-case\"><jats:italic>ftrA<\/jats:italic><\/jats:styled-content> mutant was constructed from <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009abortus<\/jats:italic> 2308. Compared with the parental strain, the <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009abortus<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\"><jats:italic>ftrA<\/jats:italic><\/jats:styled-content> mutant displays a decreased capacity to use non\u2010haem iron sources <jats:italic>in vitro<\/jats:italic>, a growth defect in a low iron medium that is enhanced at <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\u20096, and studies employing radiolabelled <jats:styled-content style=\"fixed-case\">FeCl<\/jats:styled-content><jats:sub>3<\/jats:sub> confirmed that <jats:styled-content style=\"fixed-case\">FtrABCD<\/jats:styled-content> transports ferrous iron. Transcription of the <jats:styled-content style=\"fixed-case\"><jats:italic>ftrA<\/jats:italic><\/jats:styled-content> gene is induced in <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009abortus<\/jats:italic> 2308 in response to iron deprivation and exposure to acid <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>, and similar to other <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>rucella<\/jats:italic> iron acquisition genes that have been examined the iron\u2010responsiveness of <jats:styled-content style=\"fixed-case\"><jats:italic>ftrA<\/jats:italic><\/jats:styled-content> is dependent upon the iron response regulator <jats:styled-content style=\"fixed-case\">Irr<\/jats:styled-content>. The <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009abortus<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\"><jats:italic>ftrA<\/jats:italic><\/jats:styled-content> mutant exhibits significant attenuation in both cultured murine macrophages and experimentally infected mice, supporting the proposition that ferrous iron is a critical iron source for these bacteria in the mammalian host.<\/jats:p>","DOI":"10.1111\/mmi.12242","type":"journal-article","created":{"date-parts":[[2013,5,6]],"date-time":"2013-05-06T04:10:19Z","timestamp":1367813419000},"page":"1070-1082","source":"Crossref","is-referenced-by-count":30,"title":["The ferrous iron transporter <scp>FtrABCD<\/scp> is required for the virulence of <i><scp>B<\/scp>rucella abortus<\/i> 2308 in mice"],"prefix":"10.1111","volume":"88","author":[{"given":"Ahmed E. M.","family":"Elhassanny","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology East Carolina University School of Medicine  Greenville NC 27834 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Biology East Carolina University School of Medicine  Greenville NC 27858 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan A.","family":"Menscher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology East Carolina University School of Medicine  Greenville NC 27834 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"R. 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