{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:22:27Z","timestamp":1790871747110,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T00:00:00Z","timestamp":1369180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"Summary<\/jats:title>\n \n The paralogous receptors\n PctA<\/jats:styled-content>\n ,\n PctB<\/jats:styled-content>\n and\n PctC<\/jats:styled-content>\n of\n \n P<\/jats:styled-content>\n seudomonas aeruginosa\n <\/jats:italic>\n were reported to mediate chemotaxis to amino acids, intermediates of amino acid metabolism and chlorinated hydrocarbons. We show that the recombinant ligand binding regions (\n LBRs<\/jats:styled-content>\n ) of\n PctA<\/jats:styled-content>\n ,\n PctB<\/jats:styled-content>\n and\n PctC<\/jats:styled-content>\n bind 17, 5 and 2\n l<\/jats:sc>\n \u2010amino acids respectively. In addition,\n PctC\u2010LBR<\/jats:styled-content>\n recognized\n GABA<\/jats:styled-content>\n but not any other structurally related compound.\n l<\/jats:sc>\n \u2010\n Gln<\/jats:styled-content>\n , one of the three amino acids that is not recognized by\n PctA\u2010LBR<\/jats:styled-content>\n , was the most tightly binding ligand to\n PctB<\/jats:styled-content>\n suggesting that\n PctB<\/jats:styled-content>\n has evolved to mediate chemotaxis primarily towards\n l<\/jats:sc>\n \u2010\n Gln<\/jats:styled-content>\n . Bacteria were efficiently attracted to\n l<\/jats:sc>\n \u2010\n Gln<\/jats:styled-content>\n and\n GABA<\/jats:styled-content>\n , but mutation of\n \n pctB<\/jats:italic>\n <\/jats:styled-content>\n and\n \n pctC<\/jats:italic>\n <\/jats:styled-content>\n , respectively, abolished chemoattraction. The physiological relevance of taxis towards\n GABA<\/jats:styled-content>\n is proposed to reside in an interaction with plants.\n LBRs<\/jats:styled-content>\n were predicted to adopt double\n PDC<\/jats:styled-content>\n (\n \n P<\/jats:styled-content>\n hoQ\/\n D<\/jats:styled-content>\n cuS\/\n C<\/jats:styled-content>\n itA\n <\/jats:styled-content>\n ) like structures and site\u2010directed mutagenesis studies showed that ligands bind to the membrane\u2010distal module. Analytical ultracentrifugation studies have shown that\n PctA\u2010LBR<\/jats:styled-content>\n and\n PctB\u2010LBR<\/jats:styled-content>\n are monomeric in the absence and presence of ligands, which is in contrast to the enterobacterial receptors that require sensor domain dimers for ligand recognition.\n <\/jats:p>","DOI":"10.1111\/mmi.12255","type":"journal-article","created":{"date-parts":[[2013,5,8]],"date-time":"2013-05-08T05:10:21Z","timestamp":1367989821000},"page":"1230-1243","source":"Crossref","is-referenced-by-count":110,"title":["Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non\u2010protein amino acid gamma\u2010aminobutyrate (\n GABA<\/scp>\n )"],"prefix":"10.1111","volume":"88","author":[{"given":"Miriam","family":"Rico\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Department of Environmental Protection Estaci\u00f3n Experimental del Zaid\u00edn Consejo Superior de Investigaciones Cient\u00edficas, C\/ Prof. Albareda, 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