{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:19:15Z","timestamp":1791098355210,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Autotransporters, the largest family of secreted proteins in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ram\u2010negative bacteria, perform a variety of functions, including adherence, cytotoxicity and immune evasion. In <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>ersinia pestis<\/jats:italic> the autotransporter <jats:styled-content style=\"fixed-case\">YapE<\/jats:styled-content> has adhesive properties and contributes to disease in the mouse model of bubonic plague. Here, we demonstrate that omptin cleavage of <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">YapE<\/jats:styled-content> is required to mediate bacterial aggregation and adherence to eukaryotic cells. We demonstrate that omptin cleavage is specific for the <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pseudotuberculosis<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">YapE<\/jats:styled-content> orthologues but is not conserved in the <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>ersinia enterocolitica<\/jats:italic> protein. We also show that cleavage of <jats:styled-content style=\"fixed-case\">YapE<\/jats:styled-content> occurs in <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic> but not in the enteric <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>ersinia<\/jats:italic> species, and requires the omptin <jats:styled-content style=\"fixed-case\">Pla<\/jats:styled-content> (plasminogen activator protease), which is encoded on the <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic>\u2010specific plasmid <jats:styled-content style=\"fixed-case\">pPCP<\/jats:styled-content>1. Together, these data show that post\u2010translation modification of <jats:styled-content style=\"fixed-case\">YapE<\/jats:styled-content> appears to be specific to <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic>, was acquired along with the acquisition of <jats:styled-content style=\"fixed-case\">pPCP<\/jats:styled-content>1 during the divergence of <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pestis<\/jats:italic> from <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>.\u2009pseudotuberculosis<\/jats:italic>, and are the first evidence of a novel mechanism to regulate bacterial adherence.<\/jats:p>","DOI":"10.1111\/mmi.12273","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T15:34:23Z","timestamp":1369323263000},"page":"276-287","source":"Crossref","is-referenced-by-count":17,"title":["Acquisition of omptin reveals cryptic virulence function of autotransporter <scp>YapE<\/scp> in <i><scp>Y<\/scp>ersinia pestis<\/i>"],"prefix":"10.1111","volume":"89","author":[{"given":"Matthew B.","family":"Lawrenz","sequence":"first","affiliation":[{"name":"Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases Department of Microbiology and Immunology University of Louisville School of Medicine  Louisville KY USA"},{"name":"Department of Genetics University of North Carolina School of Medicine  Chapel Hill NC USA"},{"name":"Department of Microbiology and Immunology University of North Carolina School of Medicine  Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarrod","family":"Pennington","sequence":"additional","affiliation":[{"name":"Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases Department of Microbiology and Immunology University of Louisville School of Medicine  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia L.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina School of Medicine  Chapel Hill NC USA"},{"name":"Department of Microbiology and Immunology University of North Carolina School of Medicine  Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2011.03.003"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.9.3914-3921.1993"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00458-09"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.257"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-8422-9_1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404012101"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00864-06"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.23.8172-8180.2005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093233"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02519.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn180"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.31.2.839-841.1981"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.5.1608-1615.2003"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106913200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2245-2258.2006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425909102559"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00458-10"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1231-1243.2001"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.4.692-744.2004"},{"key":"e_1_2_6_22_1","first-page":"197","article-title":"The evolution of flea\u2010borne transmission in Yersinia pestis","volume":"7","author":"Hinnebusch B.J.","year":"2005","journal-title":"Curr Issues Mol Biol"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069972"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90359-4"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.17.2281"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/317262a0"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.167650"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90478-L"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02451.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03817.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137195"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01206-08"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05877-11"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00225.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1128\/iai.56.5.1242-1248.1988","article-title":"Evidence for two genetic loci from Yersinia enterocolitica that can promote invasion of epithelial cells","volume":"56","author":"Miller V.L.","year":"1988","journal-title":"Infect Immun"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02968-06"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(18)30102-1"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-010-0600-y"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2012.02.003"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1991.tb05143.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.35"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01450-09"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.251546"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00754-09"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.5.13436"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.034991-0"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1439793"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-72124-8_24"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020206"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.59.030804.121320"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00981-10"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro730"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/09687860600927626"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.12273","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.12273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T04:52:24Z","timestamp":1696222344000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.12273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,10]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1111\/mmi.12273"],"URL":"https:\/\/doi.org\/10.1111\/mmi.12273","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,10]]}}}