{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:43:44Z","timestamp":1780472624489,"version":"3.54.1"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Connective tissues are the most common area of colonization for the <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme disease spirochaete <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia burgdorferi<\/jats:italic>. Colonization is aided by the interaction between numerous bacterial adhesins with components of the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>). Here we describe a novel interaction between <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009burgdorferi<\/jats:italic> and the major <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> proteoglycan found in joints, aggrecan. Using affinity chromatography and mass spectrometry we identify two borrelial aggrecan\u2010binding proteins: the known <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> ligand <jats:styled-content style=\"fixed-case\">Bgp<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content>0588) and an uncharacterized protease <jats:styled-content style=\"fixed-case\">BbHtrA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content>0104). Proteinase <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content> studies demonstrate that <jats:styled-content style=\"fixed-case\">BbHtrA<\/jats:styled-content> is surface exposed. Immunoblots using sera from patients with both early and late <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme disease establish that <jats:styled-content style=\"fixed-case\">BbHtrA<\/jats:styled-content> is expressed during human disease, immunogenic, and conserved in the three major <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme disease spirochaete species. Consequences of the interaction between aggrecan and <jats:styled-content style=\"fixed-case\">BbHtrA<\/jats:styled-content> were examined by proteolysis assays. <jats:styled-content style=\"fixed-case\">BbHtrA<\/jats:styled-content> cleaves aggrecan at a site known to destroy aggrecan function and which has been previously observed in the synovial fluid of patients with <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme arthritis. These data demonstrate that <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009burgdorferi<\/jats:italic> possess aggrecan\u2010binding proteins which may provide the organism with additional capability to colonize connective tissues. Moreover, our studies provide the first evidence that <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009burgdorferi<\/jats:italic> possess proteolytic activity which may contribute to the pathogenesis of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme arthritis.<\/jats:p>","DOI":"10.1111\/mmi.12276","type":"journal-article","created":{"date-parts":[[2013,5,27]],"date-time":"2013-05-27T10:18:45Z","timestamp":1369649925000},"page":"228-240","source":"Crossref","is-referenced-by-count":45,"title":["<scp>L<\/scp>yme disease spirochaetes possess an aggrecan\u2010binding protease with aggrecanase activity"],"prefix":"10.1111","volume":"90","author":[{"given":"Theresa M.","family":"Russell","sequence":"first","affiliation":[{"name":"Division of Vector\u2010Borne Diseases Centers for Disease Control and Prevention  Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara J. 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