{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:14:25Z","timestamp":1779916465880,"version":"3.53.1"},"reference-count":68,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Summary<\/jats:title>Potassium (K<\/jats:styled-content>+<\/jats:sup>) plays a vital role in bacterial physiology, including regulation of cytoplasmic pH<\/jats:styled-content>, turgor pressure and transmembrane electrical potential. Here, we examine the S<\/jats:styled-content>taphylococcus aureus<\/jats:italic>\u2005Ktr<\/jats:styled-content> system uniquely comprised of two ion\u2010conducting proteins (KtrB<\/jats:styled-content> and KtrD<\/jats:styled-content>) and only one regulator (KtrA<\/jats:styled-content>). Growth of Ktr<\/jats:styled-content> system mutants was severely inhibited under K<\/jats:styled-content>+<\/jats:sup> limitation, yet detectable after an extended lag phase, indicating the presence of a secondary K<\/jats:styled-content>+<\/jats:sup> transporter. Disruption of both ktrA<\/jats:italic><\/jats:styled-content> and the Kdp<\/jats:styled-content>\u2010ATPase<\/jats:styled-content> system, important for K<\/jats:styled-content>+<\/jats:sup> uptake in other organisms, eliminated regrowth in 0.1\u2009mM K<\/jats:styled-content>+<\/jats:sup>, demonstrating a compensatory role for Kdp<\/jats:styled-content> to the Ktr<\/jats:styled-content> system. Consistent with K<\/jats:styled-content>+<\/jats:sup> transport mutations, S<\/jats:styled-content>.\u2009aureus<\/jats:italic> devoid of the Ktr<\/jats:styled-content> system became sensitive to hyperosmotic conditions, exhibited a hyperpolarized plasma membrane, and increased susceptibility to aminoglycoside antibiotics and cationic antimicrobials. In contrast to other organisms, the S<\/jats:styled-content>.\u2009aureus<\/jats:italic>\u2005Ktr<\/jats:styled-content> system was shown to be important for low\u2010K<\/jats:styled-content>+<\/jats:sup> growth under alkaline conditions, but played only a minor role in neutral and acidic conditions. In a mouse competitive index model of bacteraemia, the ktrA<\/jats:italic><\/jats:styled-content> mutant was significantly outcompeted by the parental strain. Combined, these results demonstrate a primary mechanism of K<\/jats:styled-content>+<\/jats:sup> uptake in S<\/jats:styled-content>.\u2009aureus<\/jats:italic> and a role for this system in pathogenesis.<\/jats:p>","DOI":"10.1111\/mmi.12312","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T08:01:32Z","timestamp":1372752092000},"page":"760-773","source":"Crossref","is-referenced-by-count":81,"title":["The Ktr<\/scp> potassium transport system in S<\/scp>taphylococcus aureus<\/i> and its role in cell physiology, antimicrobial resistance and pathogenesis"],"prefix":"10.1111","volume":"89","author":[{"given":"Casey M.","family":"Gries","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center Omaha NE 68198 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology 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