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The major component is the variant surface glycoprotein (<jats:styled-content style=\"fixed-case\">VSG<\/jats:styled-content>) which is glycosylated by both paucimannose and oligomannose <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content><\/jats:italic>\u2010glycans. Surface glycans are poorly accessible and killing mediated by peptide lectin\u2013<jats:styled-content style=\"fixed-case\">VSG<\/jats:styled-content> complexes is hindered by active endocytosis. However, contrary to previous observations, here we show that high\u2010affinity carbohydrate binding agents bind to surface glycoproteins and abrogate growth of <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u2009brucei<\/jats:italic> bloodstream forms. Specifically, binding of the mannose\u2010specific <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ippeastrum<\/jats:italic> hybrid agglutinin (<jats:styled-content style=\"fixed-case\">HHA<\/jats:styled-content>) resulted in profound perturbations in endocytosis and parasite lysis. Prolonged exposure to <jats:styled-content style=\"fixed-case\">HHA<\/jats:styled-content> led to the loss of triantennary oligomannose structures in surface glycoproteins as a result of genetic rearrangements that abolished expression of the oligosaccharyltransferase <jats:styled-content style=\"fixed-case\"><jats:italic>TbSTT3B<\/jats:italic><\/jats:styled-content> gene and yielded novel chimeric enzymes. Mutant parasites exhibited markedly reduced infectivity thus demonstrating the importance of specific glycosylation patterns in parasite virulence.<\/jats:p>","DOI":"10.1111\/mmi.12359","type":"journal-article","created":{"date-parts":[[2013,8,8]],"date-time":"2013-08-08T15:22:02Z","timestamp":1375975322000},"page":"665-679","source":"Crossref","is-referenced-by-count":11,"title":["Carbohydrate\u2010binding agents act as potent trypanocidals that elicit modifications in <scp>VSG<\/scp> glycosylation and reduced virulence in <i><scp>T<\/scp>rypanosoma brucei<\/i>"],"prefix":"10.1111","volume":"90","author":[{"given":"V\u00edctor M.","family":"Castillo\u2010Acosta","sequence":"first","affiliation":[{"name":"Instituto de Parasitolog\u00eda y Biomedicina \u2018L\u00f3pez\u2010Neyra\u2019. 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