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A multi\u2010faceted peptidoglycan (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>) modification programme involving four recently characterized peptidases and two accessory proteins is essential for maintaining <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u2009pylori<\/jats:italic>'s helicity. To expedite identification of additional shape\u2010determining genes, we employed flow cytometry with fluorescence\u2010activated cell sorting (<jats:styled-content style=\"fixed-case\">FACS<\/jats:styled-content>) to enrich a transposon library for bacterial cells with altered light scattering profiles that correlate with perturbed cell morphology. After a single round of sorting, 15% of our clones exhibited a stable cell shape defect, reflecting 37\u2010fold enrichment. Sorted clones with straight rod morphology contained insertions in known <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> peptidases, as well as an insertion in <jats:styled-content style=\"fixed-case\"><jats:italic>csd6<\/jats:italic><\/jats:styled-content>, which we demonstrated has <jats:sc>ld<\/jats:sc>\u2010carboxypeptidase activity and cleaves monomeric tetrapeptides in the <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> sacculus, yielding tripeptides. Other mutants had only slight changes in helicity due to insertions in genes encoding <jats:styled-content style=\"fixed-case\">MviN<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MurJ<\/jats:styled-content>, a protein possibly involved in initiating <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> synthesis, and the hypothetical protein <jats:styled-content style=\"fixed-case\">HPG<\/jats:styled-content>27_782. Our findings demonstrate <jats:styled-content style=\"fixed-case\">FACS<\/jats:styled-content> robustly detects perturbations of bacterial cell shape and identify additional <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> peptide modifications associated with helical cell shape in <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>.\u2009pylori<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mmi.12405","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:03:22Z","timestamp":1381529002000},"page":"869-883","source":"Crossref","is-referenced-by-count":87,"title":["Flow cytometry\u2010based enrichment for cell shape mutants identifies multiple genes that influence <i><scp>H<\/scp><\/i><i>elicobacter pylori<\/i> morphology"],"prefix":"10.1111","volume":"90","author":[{"given":"Laura K.","family":"Sycuro","sequence":"first","affiliation":[{"name":"Division of Human Biology Fred Hutchinson Cancer Research Center 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