{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:05:32Z","timestamp":1777608332260,"version":"3.51.4"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T00:00:00Z","timestamp":1383868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"Summary<\/jats:title>Conserved from yeast to humans, TFIIH<\/jats:styled-content> is essential for RNA<\/jats:styled-content> polymerase II<\/jats:styled-content> transcription and nucleotide excision repair (NER<\/jats:styled-content>). TFIIH<\/jats:styled-content> consists of a core that includes the DNA<\/jats:styled-content> helicase X<\/jats:styled-content>eroderma pigmentosum<\/jats:italic>\u2005B<\/jats:styled-content> (XPB<\/jats:styled-content>) and a kinase subcomplex. T<\/jats:styled-content>rypanosoma brucei<\/jats:italic>\u2005TFIIH<\/jats:styled-content> harbours all core complex components and is indispensable for RNA<\/jats:styled-content> polymerase II<\/jats:styled-content> transcription of spliced leader RNA<\/jats:styled-content> genes (SLRNAs<\/jats:italic><\/jats:styled-content>). Kinetoplastid organisms, however, possess two highly divergent XPB<\/jats:styled-content> paralogues with only the larger being identified as a TFIIH<\/jats:styled-content> subunit in T<\/jats:styled-content>.\u2009brucei<\/jats:italic>. Here we show that a knockout of the gene for the smaller paralogue, termed XPB\u2010R<\/jats:italic><\/jats:styled-content> (R<\/jats:styled-content> for repair) resulted in viable cultured trypanosomes that grew slower than normal. XPB<\/jats:styled-content>\u2010R<\/jats:styled-content> depletion did not affect transcription in vivo<\/jats:italic> or in vitro<\/jats:italic> and XPB<\/jats:styled-content>\u2010R was not found to occupy the SLRNA<\/jats:italic><\/jats:styled-content> promoter which assembles a RNA<\/jats:styled-content> polymerase II<\/jats:styled-content> transcription pre\u2010initiation complex including TFIIH<\/jats:styled-content>. However, XPB\u2010R<\/jats:italic><\/jats:styled-content>\u2212\/\u2212<\/jats:sup> cells were much less tolerant than wild\u2010type cells to UV<\/jats:styled-content> light\u2010 and cisplatin\u2010induced DNA<\/jats:styled-content> damage, which require NER<\/jats:styled-content>. Since XPB\u2010R<\/jats:italic><\/jats:styled-content>\u2212\/\u2212<\/jats:sup> cells were not impaired in DNA<\/jats:styled-content> base excision repair, XPB<\/jats:styled-content>\u2010R<\/jats:styled-content> appears to function specifically in NER<\/jats:styled-content>. Interestingly, several other protists possess highly divergent XPB<\/jats:styled-content> paralogues suggesting that XPBs<\/jats:styled-content> specialized in transcription or NER<\/jats:styled-content> exist beyond the K<\/jats:styled-content>inetoplastida.<\/jats:p>","DOI":"10.1111\/mmi.12435","type":"journal-article","created":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T11:10:08Z","timestamp":1382094608000},"page":"1293-1308","source":"Crossref","is-referenced-by-count":15,"title":["T<\/scp>rypanosoma brucei<\/i> harbours a divergent XPB<\/scp> helicase paralogue that is specialized in nucleotide excision repair and conserved among kinetoplastid organisms"],"prefix":"10.1111","volume":"90","author":[{"given":"Nitika","family":"Badjatia","sequence":"first","affiliation":[{"name":"Department of Genetics and Developmental Biology University of Connecticut Health Center 400 Farmington Avenue Farmington CT 06030\u20106403 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