{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:14:38Z","timestamp":1792710878823,"version":"4.5.0"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,19]],"date-time":"2013-12-19T00:00:00Z","timestamp":1387411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eishmania infantum<\/jats:italic> promastigotes differentiate into amastigote forms within the phagolysosome of mammalian macrophages causing visceral leishmaniasis. Delta\u2010amastins belong to a multigenic surface protein family of potential virulence factors that are specifically expressed in the amastigote life cycle stage through distinct regulatory elements in the 3\u2032 <jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> controlling either <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> stability or translation. Here, we provide novel insights on <jats:italic>trans<\/jats:italic>\u2010acting factors regulating amastin developmental gene expression. Using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> affinity chromatography with a 300\u2009nt regulatory region within the amastin 3\u2032 <jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> as bait, we identified an Alba\u2010domain protein of 25\u2009kDa (<jats:styled-content style=\"fixed-case\"><jats:italic>Li<\/jats:italic>Alba<\/jats:styled-content>20) as a specific amastin <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\u2010binding partner. Genomic depletion of <jats:styled-content style=\"fixed-case\"><jats:italic>Li<\/jats:italic>Alba<\/jats:styled-content>20 results in amastin <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> destabilization specifically in amastigotes, supporting a role of <jats:styled-content style=\"fixed-case\"><jats:italic>Li<\/jats:italic>Alba<\/jats:styled-content>20 in amastin gene regulation. As shown by comparative <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> microarray analysis, several delta\u2010amastin transcripts but also other known developmentally regulated transcripts were downregulated in <jats:styled-content style=\"fixed-case\"><jats:italic>Li<\/jats:italic>Alba<\/jats:styled-content>20\u2212\/\u2212 knockout parasites. Inactivation of the second Alba\u2010domain gene, <jats:styled-content style=\"fixed-case\"><jats:italic>Li<\/jats:italic>Alba<\/jats:styled-content>13, does not seem to affect amastin <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> stability in either life stage of the parasite. These data indicate an important role of <jats:styled-content style=\"fixed-case\">Alba<\/jats:styled-content>\u2010domain proteins in the regulation of <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eishmania<\/jats:italic> differentially expressed transcripts and open a new field of investigation for better understanding mechanisms contributing to post\u2010transcriptional control in these parasites.<\/jats:p>","DOI":"10.1111\/mmi.12478","type":"journal-article","created":{"date-parts":[[2013,12,4]],"date-time":"2013-12-04T13:13:37Z","timestamp":1386162817000},"page":"548-561","source":"Crossref","is-referenced-by-count":45,"title":["An <scp>Alba<\/scp>\u2010domain protein contributes to the stage\u2010regulated stability of amastin transcripts in <i><scp>L<\/scp>eishmania<\/i>"],"prefix":"10.1111","volume":"91","author":[{"given":"Aur\u00e9lien","family":"Dup\u00e9","sequence":"first","affiliation":[{"name":"Research Center in Infectious Disease CHU 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