{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:02:52Z","timestamp":1791165772980,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T00:00:00Z","timestamp":1392249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Summary<\/jats:title>\n \n Contact\u2010regulated gene\n A<\/jats:styled-content>\n (\n CrgA<\/jats:styled-content>\n ) is a transcriptional regulator present in the pathogenic\n \n N<\/jats:styled-content>\n eisseria\n <\/jats:italic>\n that functions as both an activator and a repressor of transcription following contact with host cells. While its mechanism of action has been studied extensively in\n \n N<\/jats:styled-content>\n eisseria meningitidis\n <\/jats:italic>\n , the specific subset of genes that\n CrgA<\/jats:styled-content>\n targets has been debated. Although the majority of these constitute virulence genes, suggesting that\n CrgA<\/jats:styled-content>\n is important in pathogenesis, no study to date has examined the effects of\n CrgA<\/jats:styled-content>\n in\n \n N<\/jats:styled-content>\n eisseria gonorrhoeae\n <\/jats:italic>\n . In this report, we generated a knockout mutant of\n \n crgA<\/jats:italic>\n <\/jats:styled-content>\n (\u0394\n \n crgA<\/jats:italic>\n <\/jats:styled-content>\n ) in the serum\u2010sensitive gonococcal strain\n F<\/jats:styled-content>\n 62.\n \n crgA<\/jats:italic>\n <\/jats:styled-content>\n deletion resulted in a reduction in the transcript and protein levels of the primary pilin component\n \n pilE<\/jats:italic>\n <\/jats:styled-content>\n via mechanisms that were both contact\u2010dependent and \u2010independent. In contrast, \u0394\n \n crgA<\/jats:italic>\n <\/jats:styled-content>\n overexpressed the main determinant of serum resistance in\n F<\/jats:styled-content>\n 62, lipooligosaccharide sialyltransferase (\n Lst<\/jats:styled-content>\n ).\n CrgA<\/jats:styled-content>\n \u2010mediated\n \n lst<\/jats:italic>\n <\/jats:styled-content>\n repression was direct as both recombinant and native\n CrgA<\/jats:styled-content>\n bound to the\n \n lst<\/jats:italic>\n <\/jats:styled-content>\n promoter at multiple locations in\n EMSA<\/jats:styled-content>\n and\n ChIP<\/jats:styled-content>\n assays respectively. The increase in\n Lst<\/jats:styled-content>\n levels associated with\n \n crgA<\/jats:italic>\n <\/jats:styled-content>\n deletion correlated with enhanced protection against killing by normal human serum. These data suggest a role for\n CrgA<\/jats:styled-content>\n in virulence regulation during both cell adherence and planktonic growth.\n <\/jats:p>","DOI":"10.1111\/mmi.12522","type":"journal-article","created":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T02:53:20Z","timestamp":1389927200000},"page":"1120-1135","source":"Crossref","is-referenced-by-count":12,"title":["Control of pili and sialyltransferase expression in\n \n N<\/i>\n <\/scp>\n eisseria gonorrhoeae<\/i>\n is mediated by the transcriptional regulator\n CrgA<\/scp>"],"prefix":"10.1111","volume":"91","author":[{"given":"Kathryn A.","family":"Matthias","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Drexel University College of Medicine Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard F.","family":"Rest","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Drexel University College of Medicine Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,13]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff M.D.","year":"2004","journal-title":"Biophotonics Int"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.2.506"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.3.636"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1324"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.5.1068"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02838.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00212.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01439.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1128\/iai.61.5.1657-1666.1993","article-title":"Anaerobic growth and cytidine 5\u2032\u2010monophospho\u2010N\u2010acetylneuraminic acid act synergistically to induce high\u2010level serum resistance in Neisseria gonorrhoeae","volume":"61","author":"Frangipane J.V.","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1128\/iai.64.8.3374-3378.1996","article-title":"Functional characterization of a sialyltransferase\u2010deficient mutant of Neisseria gonorrhoeae","volume":"64","author":"Gill M.J.","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6938-6943.2004"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7390-7397.2005"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.6.2420-2427.1997"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02666.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3421-3430.2005"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027755-0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.85.6.1274-1279.1963"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.10.4439-4442.1992"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"4129","DOI":"10.1128\/iai.64.10.4129-4136.1996","article-title":"Expression of sialyltransferase is not required for interation of Neisseria gonorrhoeae with human epithelial cells and human neutrophils","volume":"64","author":"McGee D.J.","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4489"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1128\/iai.65.10.4341-4349.1997","article-title":"Attachment of piliated, Opa\u2212 and Opc\u2212 gonococci and meningococci to epithelial cells elicits cortical actin rearrangements and clustering of tyrosine\u2010phosphorylated proteins","volume":"65","author":"Merz A.J.","year":"1997","journal-title":"Infect Immun"},{"key":"e_1_2_6_24_1","volume-title":"Experiments in Molecular Genetics","author":"Miller J.H.","year":"1972"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6526-6534.2000"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.8.2618-2627.2003"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.2637-2650.2006"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(89)90112-5"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1992.tb05169.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06088.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02312.x"},{"key":"e_1_2_6_32_1","first-page":"49","article-title":"Function of lipopolysaccharide in the invasion of Neisseria gonorrhoeae into human mucosal cells","volume":"392","author":"Putten J.P.M.","year":"1995","journal-title":"Prog Clin Biol Res"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.5.743"},{"key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1128\/iai.60.3.989-997.1992","article-title":"Growth of Neisseria gonorrhoeae in CMP\u2010N\u2010acetylneuraminic acid inhibits nonopsonic (opacity\u2010associated outer membrane protein\u2010mediated) interactions with human neutrophils","volume":"60","author":"Rest R.F.","year":"1992","journal-title":"Infect Immun"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp445"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.6.1422"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3744-3751.2002"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.6.949"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00876.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00491.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/227382a0"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00433-06"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1696-1699.2000"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.12522","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.12522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T08:57:37Z","timestamp":1696496257000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.12522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,13]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/mmi.12522"],"URL":"https:\/\/doi.org\/10.1111\/mmi.12522","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718242137.793492749","asserted-by":"object"}]},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,13]]}}}