{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:29:49Z","timestamp":1790998189605,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,5,23]],"date-time":"2014-05-23T00:00:00Z","timestamp":1400803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM083975"],"award-info":[{"award-number":["R01GM083975"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI088298"],"award-info":[{"award-number":["R21AI088298"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI 7290775"],"award-info":[{"award-number":["DBI 7290775"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Rare lipoprotein\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    ) is a widely conserved outer membrane protein of unknown function that has previously only been studied in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>scherichia coli<\/jats:italic>\n                    , where it localizes to the septal ring and scattered foci along the lateral wall, but mutants have no phenotypic change. Here we show\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>rlpA<\/jats:italic>\n                    <\/jats:styled-content>\n                    mutants of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>seudomonas aeruginosa<\/jats:italic>\n                    form chains of short, fat cells when grown in low osmotic strength media. These morphological defects indicate\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    is needed for efficient separation of daughter cells and maintenance of rod shape. Analysis of peptidoglycan sacculi from an\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>rlpA<\/jats:italic>\n                    <\/jats:styled-content>\n                    deletion mutant revealed increased tetra and hexasaccharides that lack stem peptides (hereafter called \u2018naked glycans\u2019). Incubation of these sacculi with purified\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    resulted in release of naked glycans containing 1,6\u2010anhydro\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010acetylmuramic acid ends.\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    did not degrade sacculi from wild\u2010type cells unless the sacculi were subjected to a limited digestion with an amidase to remove some of the stem peptides. Thus,\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    is a lytic transglycosylase with a strong preference for naked glycan strands. We propose that\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    activity is regulated\n                    <jats:italic>in vivo<\/jats:italic>\n                    by substrate availability, and that amidases and\n                    <jats:styled-content style=\"fixed-case\">RlpA<\/jats:styled-content>\n                    work in tandem to degrade peptidoglycan in the division septum and lateral wall.\n                  <\/jats:p>","DOI":"10.1111\/mmi.12643","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:28:58Z","timestamp":1399508938000},"page":"113-128","source":"Crossref","is-referenced-by-count":138,"title":["The bacterial septal ring protein\n                    <scp>RlpA<\/scp>\n                    is a lytic transglycosylase that contributes to rod shape and daughter cell separation in\n                    <scp>\n                      <i>P<\/i>\n                    <\/scp>\n                    <i>seudomonas aeruginosa<\/i>"],"prefix":"10.1111","volume":"93","author":[{"given":"Matthew A.","family":"Jorgenson","sequence":"first","affiliation":[{"name":"Department of Microbiology Carver College of Medicine The University of Iowa  Iowa City IA 52242 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Virginia Tech  Blacksburg VA 24061 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Yahashiri","sequence":"additional","affiliation":[{"name":"Department of Microbiology Carver College of Medicine The University of Iowa  Iowa City IA 52242 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Popham","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Virginia Tech  Blacksburg VA 24061 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Microbiology Carver College of Medicine The University of Iowa  Iowa City IA 52242 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1039902"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01244-09"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi200085y"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08103.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03511.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi011833k"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(99)80028-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00268-13"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01270-06"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05652.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.22.7811-7814.2004"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5361.295"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12323"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300900t"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808035105"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00811-09"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.1.80-89.2004"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07445.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00127-10"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05569-11"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02499.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6093-6099.2002"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00022-11"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.124.3.1067-1076.1975"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.05.014"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2036282100"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.2"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809382105"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.13.3441-3447.1998"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3.4.S39"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja309036q"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00934-08"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511100103"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.1.558-560.1989"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.184.4.904-912.2002"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi050624n"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.61"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00425-11"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1878110"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309112044739"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06706.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00601-5"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0006"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00316-11"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18030459.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.22.6451-6458.1996"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15405"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08023.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.03.023"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00415-07"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1065"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503005102"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06376.x"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2005-6-12-242"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.03.018"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2007.06.009"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01558.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12058"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.07.067"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701818200"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.12.043"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2503"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.10.003"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00446-07"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.36"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.2.338-343.1994"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6728-6737.2004"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.18.5978-5987.2004"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00094.x"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00099.x"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.64.2.528"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq869"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107780108"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08138.x"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(88)90134-0"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.12643","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.12643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T03:40:57Z","timestamp":1696218057000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.12643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,23]]},"references-count":76,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1111\/mmi.12643"],"URL":"https:\/\/doi.org\/10.1111\/mmi.12643","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,23]]}}}