{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:59:55Z","timestamp":1791356395495,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T00:00:00Z","timestamp":1406764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004431","name":"European Commission Directorate-General for Research and Innovation","doi-asserted-by":"publisher","award":["260872"],"award-info":[{"award-number":["260872"]}],"id":[{"id":"10.13039\/100004431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004431","name":"European Commission Directorate-General for Research and Innovation","doi-asserted-by":"publisher","award":["201762"],"award-info":[{"award-number":["201762"]}],"id":[{"id":"10.13039\/100004431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The function of <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content>, a 70\u2009kDa protein in <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>ycobacterium tuberculosis<\/jats:italic>, has remained unclear. Although <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> is encoded by a gene within the <jats:styled-content style=\"fixed-case\"><jats:italic>esx\u20101<\/jats:italic><\/jats:styled-content> locus, in this study we clarify previous conflicting results and show that <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> is not essential for <jats:styled-content style=\"fixed-case\">ESX<\/jats:styled-content>\u20101\u2010mediated secretion or virulence in <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>. tuberculosis<\/jats:italic>. We also provide evidence that reduction of cellular <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> levels in wild\u2010type <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>. tuberculosis<\/jats:italic> using the drug bedaquiline completely blocks <jats:styled-content style=\"fixed-case\">ESX<\/jats:styled-content>\u20101\u2010mediated secretion. Remarkably, <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>. tuberculosis<\/jats:italic> lacking <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> fails to exhibit this phenotype. Furthermore, mutagenesis of a highly conserved <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\u2010binding motif in <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> renders <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>. tuberculosis<\/jats:italic> incapable of shutting down <jats:styled-content style=\"fixed-case\">ESX<\/jats:styled-content>\u20101\u2010mediated secretion during <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> depletion. Collectively these results show that <jats:styled-content style=\"fixed-case\"><jats:italic>M<\/jats:italic><\/jats:styled-content><jats:italic>. tuberculosis<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> negatively regulates the <jats:styled-content style=\"fixed-case\">ESX<\/jats:styled-content>\u20101 secretion system in response to low cellular <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> levels and this function requires the <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\u2010binding motif. In light of our results the potential significance of <jats:styled-content style=\"fixed-case\">EspI<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">ESX<\/jats:styled-content>\u20101 function during latent tuberculosis infection and reactivation is also discussed.<\/jats:p>","DOI":"10.1111\/mmi.12718","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T02:48:57Z","timestamp":1405478937000},"page":"1057-1065","source":"Crossref","is-referenced-by-count":32,"title":["<scp>EspI<\/scp> regulates the <scp>ESX<\/scp>\u20101 secretion system in response to <scp>ATP<\/scp> levels in <scp><i>M<\/i><\/scp><i>ycobacterium tuberculosis<\/i>"],"prefix":"10.1111","volume":"93","author":[{"given":"Ming","family":"Zhang","sequence":"first","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Chen","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sala","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Rybniker","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne Switzerland"},{"name":"1st Department of Internal Medicine University of Cologne  D\u201050937 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Dhar","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart T.","family":"Cole","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne Global Health Institute  Station 19 CH\u20101015 Lausanne 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