{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:47:22Z","timestamp":1790275642605,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"BMBF","award":["0313938A"],"award-info":[{"award-number":["0313938A"]}]},{"name":"BMBF","award":["01KI1011G"],"award-info":[{"award-number":["01KI1011G"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content><jats:italic>hlamydia trachomatis<\/jats:italic> is an obligate intracellular pathogen responsible for a high burden of human disease. Here, a loss\u2010of\u2010function screen using a set of lentivirally transduced <jats:styled-content style=\"fixed-case\">shRNAs<\/jats:styled-content> identified 14 human host cell factors that modulate <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> infectivity. Notably, knockdown of dynamin, a host <jats:styled-content style=\"fixed-case\">GTPase<\/jats:styled-content>, decreased <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> infectivity. Dynamin functions in multiple cytoplasmic locations, including vesicle formation at the plasma membrane and the trans\u2010<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>olgi network. However, its role in <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> infection remains unclear. Here we report that dynamin is essential for homotypic fusion of <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> inclusions but not for <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> internalization into the host cell. Further, dynamin activity is necessary for lipid transport into <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> inclusions and for normal re\u2010differentiation from reticulate to elementary bodies. Fragmentation of the <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>olgi apparatus is proposed to be an important strategy used by <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> for efficient lipid acquisition and replication within the host. Here we show that a subset of <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic>\u2010infected cells displayed <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>olgi fragmentation, which was concurrent with increased mitotic accumulation. <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>olgi fragmentation was dispensable for dynamin\u2010mediated lipid acquisition into <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> inclusions, irrespective of the cell cycle phase. Thus, our study reveals a critical role of dynamin in host\u2010derived lipid acquisition for <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><\/jats:styled-content>. <jats:italic>trachomatis<\/jats:italic> development.<\/jats:p>","DOI":"10.1111\/mmi.12751","type":"journal-article","created":{"date-parts":[[2014,8,13]],"date-time":"2014-08-13T13:41:51Z","timestamp":1407937311000},"page":"186-201","source":"Crossref","is-referenced-by-count":17,"title":["Dynamin\u2010mediated lipid acquisition is essential for <scp><i>C<\/i><\/scp><i>hlamydia trachomatis<\/i> development"],"prefix":"10.1111","volume":"94","author":[{"given":"Rajendra Kumar","family":"Gurumurthy","sequence":"first","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindrilla","family":"Chumduri","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Karlas","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Kimmig","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus","family":"Machuy","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology Max Planck Institute for Infection Biology  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