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Two primary virulence factors of <jats:styled-content style=\"fixed-case\"><jats:italic>F<\/jats:italic><\/jats:styled-content><jats:italic>. magna<\/jats:italic> are the subtilisin\u2010like extracellular serine protease <jats:styled-content style=\"fixed-case\">SufA<\/jats:styled-content> and the adhesive protein <jats:styled-content style=\"fixed-case\">FAF<\/jats:styled-content>. This study examines the molecular mechanisms <jats:styled-content style=\"fixed-case\"><jats:italic>F<\/jats:italic><\/jats:styled-content><jats:italic>. magna<\/jats:italic> uses when colonizing or establishing an infection in the skin. <jats:styled-content style=\"fixed-case\">FAF<\/jats:styled-content> was found to be essential in the initial adherence of <jats:styled-content style=\"fixed-case\"><jats:italic>F<\/jats:italic><\/jats:styled-content><jats:italic>. magna<\/jats:italic> to human skin biopsies. In the upper layers of the epidermis <jats:styled-content style=\"fixed-case\">FAF<\/jats:styled-content> mediates adhesion through binding to galectin\u20107 \u2013 a keratinocyte cell marker. Once the bacteria moved deeper into the skin to the basement membrane layer, <jats:styled-content style=\"fixed-case\">SufA<\/jats:styled-content> was found to degrade collagen IV which forms the backbone structure of the basement membrane. It also degraded collagen V, whereby <jats:styled-content style=\"fixed-case\"><jats:italic>F<\/jats:italic><\/jats:styled-content><jats:italic>. magna<\/jats:italic> could reach deeper dermal tissue sites. In the dermis, <jats:styled-content style=\"fixed-case\">FAF<\/jats:styled-content> interacts with collagen V and fibrillin, which presumably helps the bacteria to establish infection in this area. The findings of this study paint a clear picture of how <jats:styled-content style=\"fixed-case\"><jats:italic>F<\/jats:italic><\/jats:styled-content><jats:italic>. magna<\/jats:italic> interacts with human skin and explain how it is such a successful opportunistic pathogen in chronic wounds and ulcers.<\/jats:p>","DOI":"10.1111\/mmi.12773","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T03:54:07Z","timestamp":1409198047000},"page":"403-417","source":"Crossref","is-referenced-by-count":42,"title":["Identification of molecular mechanisms used by <scp><i>F<\/i><\/scp><i>inegoldia magna<\/i> to penetrate and colonize human skin"],"prefix":"10.1111","volume":"94","author":[{"given":"Elizabeth C.","family":"Murphy","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Division of Infection Medicine Lund University  SE\u201022184 Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"M\u00f6rgelin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Division of Infection Medicine Lund University  SE\u201022184 Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter P.","family":"Reinhardt","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Faculty of Medicine and Faculty of Dentistry Division of Biomedical Sciences McGill University  Montreal QC H3A 2B2 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders I.","family":"Olin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Division of Infection Medicine Lund University  SE\u201022184 Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Bj\u00f6rck","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Division of Infection Medicine Lund University  SE\u201022184 Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga\u2010Maria","family":"Frick","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Division of Infection Medicine Lund University  SE\u201022184 Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1060140405"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.999"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.4049\/jimmunol.140.4.1194","article-title":"Protein L. 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