{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:57:33Z","timestamp":1791147453412,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T00:00:00Z","timestamp":1410998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-07-BLAN-0106-02"],"award-info":[{"award-number":["ANR-07-BLAN-0106-02"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Summary<\/jats:title>\n \n \n D<\/jats:italic>\n <\/jats:styled-content>\n einococcus<\/jats:italic>\n bacteria are famous for their extreme radiation tolerance. The\n IrrE<\/jats:styled-content>\n protein was shown to be essential for radiation tolerance and, in an unelucidated manner, for induction of a number of genes in response to radiation, including\n \n recA<\/jats:italic>\n <\/jats:styled-content>\n and other\n DNA<\/jats:styled-content>\n repair genes. Earlier studies indicated that\n IrrE<\/jats:styled-content>\n could be a zinc peptidase, but proteolytic activity was not demonstrated. Here, using several\n in vivo<\/jats:italic>\n and\n in vitro<\/jats:italic>\n experiments,\n IrrE<\/jats:styled-content>\n from\n \n D<\/jats:italic>\n <\/jats:styled-content>\n einococcus deserti<\/jats:italic>\n was found to interact with\n DdrO<\/jats:styled-content>\n , a predicted regulator encoded by a radiation\u2010induced gene that is, like\n \n irrE<\/jats:italic>\n <\/jats:styled-content>\n , highly conserved in\n \n D<\/jats:italic>\n <\/jats:styled-content>\n einococcus<\/jats:italic>\n . Moreover,\n IrrE<\/jats:styled-content>\n was found to cleave\n DdrO<\/jats:styled-content>\n \u2005\n in vitro<\/jats:italic>\n and when the proteins were coexpressed in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n scherichia coli<\/jats:italic>\n . This cleavage was not observed in the presence of metal chelator\n EDTA<\/jats:styled-content>\n or when\n IrrE<\/jats:styled-content>\n contains a mutation in the conserved active\u2010site motif of metallopeptidases. In\n \n D<\/jats:italic>\n <\/jats:styled-content>\n . deserti<\/jats:italic>\n ,\n IrrE<\/jats:styled-content>\n \u2010dependent cleavage of\n DdrO<\/jats:styled-content>\n was observed after exposure to radiation. Furthermore,\n DdrO<\/jats:styled-content>\n \u2010dependent repression of the promoter of a radiation\u2010induced gene was shown. These results demonstrate that\n IrrE<\/jats:styled-content>\n is a metalloprotease and we propose that\n IrrE<\/jats:styled-content>\n \u2010mediated cleavage inactivates repressor protein\n DdrO<\/jats:styled-content>\n , leading to transcriptional induction of various genes required for repair and survival after exposure of\n \n D<\/jats:italic>\n <\/jats:styled-content>\n einococcus<\/jats:italic>\n to radiation.\n <\/jats:p>","DOI":"10.1111\/mmi.12774","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T05:53:52Z","timestamp":1409291632000},"page":"434-449","source":"Crossref","is-referenced-by-count":92,"title":["Radiation response in\n \n D<\/i>\n <\/scp>\n einococcus deserti<\/i>\n :\n IrrE<\/scp>\n is a metalloprotease that cleaves repressor protein\n DdrO<\/scp>"],"prefix":"10.1111","volume":"94","author":[{"given":"Monika","family":"Ludanyi","sequence":"first","affiliation":[{"name":"CEA, DSV, IBEB Lab Bioenerget Cellulaire 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pignol","sequence":"additional","affiliation":[{"name":"CEA, DSV, IBEB Lab Bioenerget Cellulaire Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 France"},{"name":"CNRS UMR 7265 Biol Veget & Microbiol Environ Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 France"},{"name":"Aix\u2010Marseille Universit\u00e9 Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lemaire","sequence":"additional","affiliation":[{"name":"CNRS UMR 7265 Biol Veget & Microbiol Environ Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 France"},{"name":"Aix\u2010Marseille Universit\u00e9 Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 France"},{"name":"CEA, DSV, IBEB Lab Interact Protein Metal Saint\u2010Paul\u2010lez\u2010Durance F\u201013108 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