{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:25:30Z","timestamp":1790364330223,"version":"4.1.0"},"reference-count":81,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T00:00:00Z","timestamp":1412035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00c3\u00a7\u00c3\u00a3o de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Summary<\/jats:title>A<\/jats:italic><\/jats:styled-content>spergillus fumigatus<\/jats:italic> is an opportunistic pathogen and allergen of mammals. Calcium signalling is essential for A<\/jats:italic><\/jats:styled-content>. fumigatus<\/jats:italic> pathogenicity and is regulated by the CrzA<\/jats:styled-content> transcription factor. We used ChIP<\/jats:styled-content>\u2010seq (C<\/jats:styled-content>hromatin I<\/jats:styled-content>mmunoprecipitation DNA<\/jats:styled-content> sequencing) to explore CrzA<\/jats:styled-content> gene targets in A<\/jats:italic><\/jats:styled-content>. fumigatus<\/jats:italic>. In total, 165 potential binding peaks including 102 directly regulated genes were identified, resulting in the prediction of the A<\/jats:styled-content>[GT<\/jats:styled-content>][CG<\/jats:styled-content>]CA<\/jats:styled-content>[AC<\/jats:styled-content>][AG<\/jats:styled-content>] CrzA<\/jats:styled-content>\u2010binding motif. The 102 CrzA<\/jats:styled-content> putatively regulated genes exhibited a diverse array of functions. The phkB<\/jats:italic><\/jats:styled-content> (A<\/jats:styled-content>fu3g12530) histidine kinase and the sskB<\/jats:italic><\/jats:styled-content> (A<\/jats:styled-content>fu1g10940) MAP<\/jats:styled-content> kinase kinase kinase of the HOG<\/jats:styled-content> (high\u2010osmolarity glycerol response) pathway were regulated by CrzA<\/jats:styled-content>. Several members of the two\u2010component system (TCS<\/jats:styled-content>) and the HOG<\/jats:styled-content> pathway were more sensitive to calcium. CrzA<\/jats:styled-content>::GFP<\/jats:styled-content> was translocated to the nucleus upon osmotic stress. CrzA<\/jats:styled-content> is important for the phosphorylation of the SakA MAPK<\/jats:styled-content> in response to osmotic shock. The \u0394sskB<\/jats:italic><\/jats:styled-content> was more sensitive to CaCl<\/jats:styled-content>2<\/jats:sub>, NaCl<\/jats:styled-content>, and paraquat stress, while being avirulent in a murine model of invasive pulmonary aspergillosis. The presence of CaCl<\/jats:styled-content>2<\/jats:sub> and osmotic stresses resulted in synergistic inhibition of \u0394crzA<\/jats:italic><\/jats:styled-content> and \u0394sskB<\/jats:italic><\/jats:styled-content> growth. These results suggest there is a genetic interaction between the A. fumigatus<\/jats:italic> calcineurin\u2010CrzA<\/jats:styled-content> and HOG<\/jats:styled-content> pathway that is essential for full virulence.<\/jats:p>","DOI":"10.1111\/mmi.12785","type":"journal-article","created":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T08:09:29Z","timestamp":1409904569000},"page":"655-674","source":"Crossref","is-referenced-by-count":49,"title":["ChIP<\/scp>\u2010seq reveals a role for CrzA<\/scp> in the A<\/i><\/scp>spergillus fumigatus<\/i> high\u2010osmolarity glycerol response (HOG<\/scp>) signalling pathway"],"prefix":"10.1111","volume":"94","author":[{"given":"Patr\u00edcia A.","family":"de Castro","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo Ribeir\u00e3o Preto 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