{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T16:30:37Z","timestamp":1769099437468,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T00:00:00Z","timestamp":1412553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FU375\/7-1, FU375\/8-1"],"award-info":[{"award-number":["FU375\/7-1, FU375\/8-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Summary<\/jats:title>In S<\/jats:italic><\/jats:styled-content>almonella enterica<\/jats:italic> serovar T<\/jats:styled-content>yphimurium (S<\/jats:italic><\/jats:styled-content>. T<\/jats:styled-content>yphimurium), the genomic island GEI<\/jats:styled-content>4417\/4436 is responsible for the utilization of myo<\/jats:italic>\u2010inositol (MI<\/jats:styled-content>) as carbon and energy source. Here, we report the characterization of a novel, island\u2010encoded positive autoregulator termed ReiD<\/jats:styled-content> (STM<\/jats:styled-content>4423) that is specific to certain S<\/jats:italic><\/jats:styled-content>. enterica<\/jats:italic> strains and E<\/jats:italic><\/jats:styled-content>scherichia coli<\/jats:italic> strain ED<\/jats:styled-content>1a able to use MI<\/jats:styled-content>. ReiD<\/jats:styled-content> was essential for growth with this polyol and also contributed to S<\/jats:italic><\/jats:styled-content>. T<\/jats:styled-content>yphimurium proliferation in swine caecum content. Providing higher copy numbers of ReiD<\/jats:styled-content> reduced the long lag phase of 2 days during growth of S<\/jats:italic><\/jats:styled-content>. T<\/jats:styled-content>yphimurium in MI<\/jats:styled-content> medium by 50%. In a heterologous host, expression of ReiD<\/jats:styled-content> activated the transcription from the promoter of iolE<\/jats:italic><\/jats:styled-content>\/iolG<\/jats:italic><\/jats:styled-content>, whose products catalyse the initial two steps in MI<\/jats:styled-content> degradation. Episomal expression of iolE<\/jats:italic><\/jats:styled-content>\/iolG1<\/jats:italic><\/jats:styled-content> rescued the otherwise zero growth phenotype of a reiD<\/jats:italic><\/jats:styled-content> deletion mutant in MI<\/jats:styled-content> medium. Gel mobility shift assays with purified ReiD<\/jats:styled-content> demonstrated directed interaction of ReiD<\/jats:styled-content> with its own promoter and that of iolE<\/jats:italic><\/jats:styled-content>. The repressor IolR<\/jats:styled-content> bound the reiD<\/jats:italic><\/jats:styled-content> promoter, implying that reiD<\/jats:italic><\/jats:styled-content> is part of the IolR<\/jats:styled-content> regulon. Taken together, the regulator ReiD<\/jats:styled-content> is a trigger to accelerate the switch from more easily accessible nutrients to MI<\/jats:styled-content> utilization by S<\/jats:italic><\/jats:styled-content>. T<\/jats:styled-content>yphimurium.<\/jats:p>","DOI":"10.1111\/mmi.12788","type":"journal-article","created":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T02:53:02Z","timestamp":1410490382000},"page":"700-712","source":"Crossref","is-referenced-by-count":17,"title":["The orphan regulator ReiD<\/scp> of S<\/i><\/scp>almonella enterica<\/i> is essential for myo<\/i>\u2010inositol utilization"],"prefix":"10.1111","volume":"94","author":[{"given":"Johannes E.","family":"Rothhardt","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobielle \u00d6kologie Zentralinstitut f\u00fcr Ern\u00e4hrungs\u2010 und Lebensmittelforschung (ZIEL) Wissenschaftszentrum Weihenstephan Technische Universit\u00e4t M\u00fcnchen Weihenstephaner Berg 3 85354 Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Kr\u00f6ger","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobielle \u00d6kologie Zentralinstitut f\u00fcr Ern\u00e4hrungs\u2010 und Lebensmittelforschung (ZIEL) Wissenschaftszentrum Weihenstephan Technische Universit\u00e4t M\u00fcnchen Weihenstephaner Berg 3 85354 Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven P.","family":"Broadley","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobielle \u00d6kologie Zentralinstitut f\u00fcr Ern\u00e4hrungs\u2010 und Lebensmittelforschung (ZIEL) Wissenschaftszentrum Weihenstephan Technische Universit\u00e4t M\u00fcnchen Weihenstephaner Berg 3 85354 Freising Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thilo M.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Lehrstuhl f\u00fcr Mikrobielle \u00d6kologie Zentralinstitut f\u00fcr Ern\u00e4hrungs\u2010 und Lebensmittelforschung (ZIEL) Wissenschaftszentrum Weihenstephan Technische Universit\u00e4t M\u00fcnchen Weihenstephaner Berg 3 85354 Freising 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