{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:02:56Z","timestamp":1791414176981,"version":"4.3.3"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,10,19]],"date-time":"2014-10-19T00:00:00Z","timestamp":1413676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI107792"],"award-info":[{"award-number":["U19AI107792"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI107159"],"award-info":[{"award-number":["R01AI107159"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Summary<\/jats:title>\n \n In the intracellular pathogen\n \n B<\/jats:italic>\n <\/jats:styled-content>\n rucella abortus<\/jats:italic>\n , the general stress response (\n GSR<\/jats:styled-content>\n ) signalling system determines survival under acute stress conditions\n in vitro<\/jats:italic>\n , and is required for long\u2010term residence in a mammalian host. To date, the identity of the\n \n B<\/jats:italic>\n <\/jats:styled-content>\n rucella<\/jats:italic>\n sensor kinase(s) that function to perceive stress and directly activate\n GSR<\/jats:styled-content>\n signalling have remained undefined. We demonstrate that the flavin\u2010binding sensor histidine kinase,\n LovhK<\/jats:styled-content>\n (\n \n bab2_0652<\/jats:italic>\n <\/jats:styled-content>\n ), functions as a primary\n \n B<\/jats:italic>\n <\/jats:styled-content>\n . abortus<\/jats:italic>\n \u2005\n GSR<\/jats:styled-content>\n sensor.\n LovhK<\/jats:styled-content>\n rapidly and specifically phosphorylates the central\n GSR<\/jats:styled-content>\n regulator,\n PhyR<\/jats:styled-content>\n , and activates transcription of a set of genes that closely overlaps the known\n \n B<\/jats:italic>\n <\/jats:styled-content>\n . abortus<\/jats:italic>\n \u2005\n GSR<\/jats:styled-content>\n regulon. Deletion of\n \n lovhK<\/jats:italic>\n <\/jats:styled-content>\n severely compromises cell survival under defined oxidative and acid stress conditions. We further show that\n \n lovhK<\/jats:italic>\n <\/jats:styled-content>\n is required for cell survival during the early phase of mammalian cell infection and for establishment of long\u2010term residence in a mouse infection model. Finally, we present evidence that particular regions of primary structure within the two\n N<\/jats:styled-content>\n \u2010terminal\n PAS<\/jats:styled-content>\n domains of\n LovhK<\/jats:styled-content>\n have distinct sensory roles under specific environmental conditions. This study elucidates new molecular components of a conserved signalling pathway that regulates\n \n B<\/jats:italic>\n <\/jats:styled-content>\n . abortus<\/jats:italic>\n stress physiology and infection biology.\n <\/jats:p>","DOI":"10.1111\/mmi.12809","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T04:44:29Z","timestamp":1411706669000},"page":"913-925","source":"Crossref","is-referenced-by-count":57,"title":["The\n \n B<\/i>\n <\/scp>\n rucella abortus<\/i>\n virulence regulator,\n LovhK<\/scp>\n , is a sensor kinase in the general stress response signalling pathway"],"prefix":"10.1111","volume":"94","author":[{"given":"Hye\u2010Sook","family":"Kim","sequence":"first","affiliation":[{"name":"The Department of Biochemistry and Molecular Biology University of Chicago Chicago IL USA"},{"name":"Howard Taylor Ricketts Laboratory University of Chicago, Argonne National Laboratory Argonne IL 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Crosson","sequence":"additional","affiliation":[{"name":"The Department of Biochemistry and Molecular Biology University of Chicago Chicago IL USA"},{"name":"The Committee on Microbiology University of Chicago Chicago IL USA"},{"name":"Howard Taylor Ricketts Laboratory University of Chicago, Argonne National Laboratory Argonne IL 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