{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:43:12Z","timestamp":1790520192754,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T00:00:00Z","timestamp":1419897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["3016\/1-1"],"award-info":[{"award-number":["3016\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Deutsches Zentrum f\u00c3\u00bcr Infektionsforschung","award":["TTU 07.801"],"award-info":[{"award-number":["TTU 07.801"]}]},{"name":"M\u00c3\u00bcnchner Medizinische Wochenschrift"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Echinocandins inhibit \u03b2\u20101,3\u2010glucan synthesis and are one of the few antimycotic drug classes effective against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>spergillus spp.<\/jats:italic>\n                    In this study, we characterized the \u03b2\u20101,3\u2010glucan synthase\n                    <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                    ks1 of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>spergillus fumigatus<\/jats:italic>\n                    , the putative target of echinocandins. Data obtained with a conditional mutant suggest that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>fks1<\/jats:italic>\n                    <\/jats:styled-content>\n                    is not essential. In agreement, we successfully constructed a viable \u0394\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>fks1<\/jats:italic>\n                    <\/jats:styled-content>\n                    deletion mutant. Lack of\n                    <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                    ks1 results in characteristic growth phenotypes similar to wild type treated with echinocandins and an increased susceptibility to calcofluor white and sodium dodecyl sulfate. In agreement with\n                    <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                    ks1 being the only \u03b2\u20101,3\u2010glucan synthase in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>.\u2009fumigatus<\/jats:italic>\n                    , the cell wall is devoid of \u03b2\u20101,3\u2010glucan. This is accompanied by a compensatory increase of chitin and galactosaminogalactan and a significant decrease in cell wall galactomannan due to a massively enhanced galactomannan shedding. Our data furthermore suggest that inhibition of hyphal septation can overcome the limitations of echinocandin therapy. Compounds inhibiting septum formation boosted the antifungal activity of caspofungin. Thus, development of clinically applicable inhibitors of septum formation is a promising strategy to improve existing antifungal therapy.\n                  <\/jats:p>","DOI":"10.1111\/mmi.12877","type":"journal-article","created":{"date-parts":[[2014,11,25]],"date-time":"2014-11-25T21:29:49Z","timestamp":1416950989000},"page":"458-471","source":"Crossref","is-referenced-by-count":102,"title":["<scp>\n                      <i>A<\/i>\n                    <\/scp>\n                    <i>spergillus fumigatus<\/i>\n                    devoid of cell wall \u03b2\u20101,3\u2010glucan is viable, massively sheds galactomannan and is killed by septum formation inhibitors"],"prefix":"10.1111","volume":"95","author":[{"given":"Karl","family":"Dichtl","sequence":"first","affiliation":[{"name":"Max von Pettenkofer\u2010Institut f\u00fcr Hygiene und Medizinische Mikrobiologie Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  80336 Munich 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