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In contrast, in prokaryotes, deficiency in the\n FXN<\/jats:styled-content>\n homolog,\n CyaY<\/jats:styled-content>\n , was reported not to cause any significant phenotype, questioning both its importance and its actual contribution to\n F<\/jats:styled-content>\n e\u2010\n S<\/jats:styled-content>\n cluster biogenesis. Because\n FXN<\/jats:styled-content>\n is conserved between eukaryotes and prokaryotes, this surprising discrepancy prompted us to reinvestigate the role of\n CyaY<\/jats:styled-content>\n in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n scherichia coli<\/jats:italic>\n . We report that\n CyaY<\/jats:styled-content>\n (i) potentiates\n \n E<\/jats:italic>\n <\/jats:styled-content>\n .\u2009coli<\/jats:italic>\n fitness, (ii) belongs to the\n ISC<\/jats:styled-content>\n pathway catalyzing the maturation of\n F<\/jats:styled-content>\n e\u2010\n S<\/jats:styled-content>\n cluster\u2010containing proteins and (iii) requires iron\u2010rich conditions for its contribution to be significant. A genetic interaction was discovered between\n \n cyaY<\/jats:italic>\n <\/jats:styled-content>\n and\n \n iscX<\/jats:italic>\n <\/jats:styled-content>\n , the last gene of the\n \n isc<\/jats:italic>\n <\/jats:styled-content>\n operon. Deletion of both genes showed an additive effect on\n F<\/jats:styled-content>\n e\u2010\n S<\/jats:styled-content>\n cluster protein maturation, which led, among others, to increased resistance to aminoglycosides and increased sensitivity to lambda phage infection. Together, these\n in vivo<\/jats:italic>\n results establish the importance of\n CyaY<\/jats:styled-content>\n as a member of the\n ISC<\/jats:styled-content>\n \u2010mediated\n F<\/jats:styled-content>\n e\u2010\n S<\/jats:styled-content>\n cluster biogenesis pathway in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n .\u2009coli<\/jats:italic>\n , like it does in eukaryotes, and validate\n IscX<\/jats:styled-content>\n as a new bona fide\n F<\/jats:styled-content>\n e\u2010\n S<\/jats:styled-content>\n cluster biogenesis factor.\n <\/jats:p>","DOI":"10.1111\/mmi.12888","type":"journal-article","created":{"date-parts":[[2014,11,28]],"date-time":"2014-11-28T10:51:32Z","timestamp":1417171892000},"page":"605-623","source":"Crossref","is-referenced-by-count":61,"title":["The iron\u2010binding\n CyaY<\/scp>\n and\n IscX<\/scp>\n proteins assist the\n ISC<\/scp>\n \u2010catalyzed\n F<\/scp>\n e\u2010\n S<\/scp>\n biogenesis in\n \n E<\/i>\n <\/scp>\n scherichia 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13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Py","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne UMR 7283 Aix\u2010Marseille Universit\u00e9\u2010CNRS Institut de Microbiologie de la M\u00e9diterran\u00e9e 31 Chemin Joseph Aiguier 13009 Marseille 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