{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:24:32Z","timestamp":1790364272952,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T00:00:00Z","timestamp":1423612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>A<\/jats:italic><\/jats:styled-content><jats:italic>spergillus fumigatus<\/jats:italic> is a fungal pathogen that is capable of adapting to different host niches and to avoid host defenses. An enhanced understanding of how, and which, <jats:styled-content style=\"fixed-case\"><jats:italic>A<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009fumigatus<\/jats:italic> signal transduction pathways are engaged in the regulation of these processes is essential for the development of improved disease control strategies. Protein phosphatases are central to numerous signal transduction pathways. To comprehend the functions of protein phosphatases in <jats:styled-content style=\"fixed-case\"><jats:italic>A<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009fumigatus<\/jats:italic>, 32 phosphatase catalytic subunit encoding genes were identified. We have recognized <jats:styled-content style=\"fixed-case\">PtcB<\/jats:styled-content> as one of the phosphatases involved in the high osmolarity glycerol response (HOG) pathway. The <jats:styled-content style=\"fixed-case\"><jats:italic>\u0394ptcB<\/jats:italic><\/jats:styled-content> mutant has both increased phosphorylation of the p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">SakA<\/jats:styled-content>) and expression of osmo\u2010dependent genes. The <jats:styled-content style=\"fixed-case\"><jats:italic>\u0394ptcB<\/jats:italic><\/jats:styled-content> strain was more sensitive to cell wall damaging agents, had increased chitin and <jats:italic>\u03b2<\/jats:italic>\u20101,3\u2010glucan, and impaired biofilm formation. The <jats:styled-content style=\"fixed-case\"><jats:italic>\u0394ptcB<\/jats:italic><\/jats:styled-content> strain was avirulent in a murine model of invasive pulmonary aspergillosis. These results stress the importance of the <jats:styled-content style=\"fixed-case\">HOG<\/jats:styled-content> pathway in the regulation of pathogenicity determinants and virulence in <jats:styled-content style=\"fixed-case\"><jats:italic>A<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009fumigatus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mmi.12919","type":"journal-article","created":{"date-parts":[[2015,1,19]],"date-time":"2015-01-19T09:36:22Z","timestamp":1421660182000},"page":"42-54","source":"Crossref","is-referenced-by-count":54,"title":["<scp>H<\/scp>igh osmolarity glycerol response <scp>PtcB<\/scp> phosphatase is important for <scp><i>A<\/i><\/scp><i>spergillus fumigatus<\/i> virulence"],"prefix":"10.1111","volume":"96","author":[{"given":"Lizziane K.","family":"Winkelstr\u00f6ter","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Leite Pedro","family":"Bom","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Alves","family":"de Castro","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandra Naira Zambelli","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Helena S.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Filosofia Ci\u00eancias e Letras de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil Andrew","family":"Brown","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjith","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Infection and Immunity Research Group Glasgow Dental School School of Medicine College of Medical Veterinary and Life Sciences The University of Glasgow  378 Sauchiehall Street Glasgow G2\u20093JZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Ramage","sequence":"additional","affiliation":[{"name":"Infection and Immunity Research Group Glasgow Dental School School of Medicine College of Medical Veterinary and Life Sciences The University of Glasgow  378 Sauchiehall Street Glasgow G2\u20093JZ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Bovier","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaila Fernanda","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Savoldi","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Hagiwara","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"},{"name":"National Laboratory of Science and Technology of Bioethanol (CTBE)  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.057075-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.21.7117-7136.2001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00249-10"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00323-08"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176495"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005458"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034508329273"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12626"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601456103"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.48"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00055-08"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06747.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037591"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-012-0385-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-20-8-0944"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6428.2004"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1778"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.008813"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.1.34-48.2003"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M607919200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2006.05.013"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.130179"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080881"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.096156"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00654.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.28.17749"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025311"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(08)60245-X"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00069-14"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-012-9600-5"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04822.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.11.7651"},{"key":"e_1_2_6_35_1","first-page":"121","volume-title":"The Aspergilli. Genomics, Medical Aspects, Biotechnology, and Research Methods","author":"May G.S.","year":"2008"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28616-0"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2126"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081986"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47247-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2014.02.009"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115647"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.22.2.153"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00178-06"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.01.002"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.02.001"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.140863"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvh135"},{"key":"e_1_2_6_48_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_6_49_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(00)00694-6"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00340712"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2012.11.010"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.10.006"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12023"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00141"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00346-08"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1680"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.09.006"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.08.005"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.1.51-60.2001"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2003.11.031"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-11-0914"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.3.1289"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.2.557-560.2004"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061307"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12126"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12776"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.375"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-2734-4.50017-6"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.12919","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.12919","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T22:10:51Z","timestamp":1696198251000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.12919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,11]]},"references-count":69,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1111\/mmi.12919"],"URL":"https:\/\/doi.org\/10.1111\/mmi.12919","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,11]]}}}