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Previous studies indicate that a putative lipoprotein, BBA<\/jats:styled-content>33, is produced in an RpoS<\/jats:styled-content>\u2010dependent manner under conditions that mimic the mammalian component of the borrelial lifecycle. However, the significance and function for BBA<\/jats:styled-content>33 is not known. Given its linkage to the BosR\/Rrp2\u2010RpoN\u2010RpoS<\/jats:styled-content> regulatory cascade, we hypothesized that BBA<\/jats:styled-content>33 facilitates B<\/jats:italic><\/jats:styled-content>.\u2009burgdorferi<\/jats:italic> infection in the mammalian host. The deletion of bba33<\/jats:italic> eliminated B<\/jats:italic><\/jats:styled-content>.\u2009burgdorferi<\/jats:italic> infectivity in C<\/jats:styled-content>3H<\/jats:styled-content> mice, which was rescued by genetic complementation with intact bba33<\/jats:italic>. With regard to function, a combinatorial peptide approach, coupled with subsequent in vitro<\/jats:italic> binding assays, indicated that BBA<\/jats:styled-content>33 binds to collagen type VI<\/jats:styled-content> and, to a lesser extent, collagen type IV<\/jats:styled-content>. Whole cell binding assays demonstrated BBA<\/jats:styled-content>33\u2010dependent binding to human collagen type VI<\/jats:styled-content>. Taken together, these results suggest that BBA<\/jats:styled-content>33 interacts with collagenous structures and may function as an adhesin in a process that is required to prevent bacterial clearance.<\/jats:p>","DOI":"10.1111\/mmi.12921","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T07:13:45Z","timestamp":1420528425000},"page":"68-83","source":"Crossref","is-referenced-by-count":21,"title":["The BBA<\/scp>33 lipoprotein binds collagen and impacts B<\/i><\/scp>orrelia burgdorferi<\/i> pathogenesis"],"prefix":"10.1111","volume":"96","author":[{"given":"Hui","family":"Zhi","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology College of Medicine Texas A&M Health Science Center Bryan TX 77807 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric H.","family":"Weening","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology College of Medicine Texas A&M Health Science Center Bryan TX 77807 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena Magda","family":"Barbu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The University of Texas M. 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