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This dispersal occurred via a mechanism that was dependent upon expression of both <jats:styled-content style=\"fixed-case\">PilA<\/jats:styled-content> (and by inference, Tfp) and production of <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\u20102 quorum signaling molecules by <jats:styled-content style=\"fixed-case\">LuxS<\/jats:styled-content>. The addition of <jats:styled-content style=\"fixed-case\">rsPilA<\/jats:styled-content> to a biofilm\u2010targeted therapeutic vaccine formulation comprised of <jats:styled-content style=\"fixed-case\">IHF<\/jats:styled-content> plus the powerful adjuvant <jats:styled-content style=\"fixed-case\">dmLT<\/jats:styled-content> and delivered via a noninvasive transcutaneous immunization route induced an immune response that targeted two important determinants essential for biofilm formation by <jats:styled-content style=\"fixed-case\">NTHI<\/jats:styled-content>. This resulted in significantly earlier eradication of <jats:styled-content style=\"fixed-case\">NTHI<\/jats:styled-content> from both planktonic and adherent populations in the middle ear, disruption of mucosal biofilms already resident within middle ears prior to immunization and rapid resolution of signs of disease in an animal model of experimental otitis media. These data support continued development of this novel combinatorial immunization approach for resolution and\/or prevention of multiple diseases of the respiratory tract caused by <jats:styled-content style=\"fixed-case\">NTHI<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/mmi.12934","type":"journal-article","created":{"date-parts":[[2015,1,19]],"date-time":"2015-01-19T09:35:40Z","timestamp":1421660140000},"page":"276-292","source":"Crossref","is-referenced-by-count":64,"title":["Antibodies against the majority subunit of type <scp>IV<\/scp> pili disperse nontypeable <scp><i>H<\/i><\/scp><i>aemophilus influenzae<\/i> biofilms in a <scp>LuxS<\/scp>\u2010dependent manner and confer therapeutic resolution of experimental otitis media"],"prefix":"10.1111","volume":"96","author":[{"given":"Laura A.","family":"Novotny","sequence":"first","affiliation":[{"name":"Center for Microbial Pathogenesis The Research Institute at Nationwide Children's Hospital  700 Children's Drive Columbus OH 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