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We show that this synergistic toxicity is associated with catastrophic chromosomal fragmentation. Since <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> alone does not kill at any concentration, while <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> alone kills at 20\u2009mM, <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> must potentiate <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> poisoning. The <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>\u2009+\u2009<jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> killing is blocked by iron chelators, suggesting Fenton's reaction. Indeed, we show that <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> enhances plasmid <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> relaxation due to Fenton's reaction <jats:italic>in vitro<\/jats:italic>. However, mutants with elevated iron or <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> pools are not acutely sensitive to <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>\u2010alone treatment, suggesting that, in addition, <jats:italic>in vivo<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content> recruits iron from intracellular depots. We found that part of the <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>\u2010recruited iron pool is managed by ferritin and <jats:styled-content style=\"fixed-case\">Dps<\/jats:styled-content>: ferritin releases iron on cue from <jats:styled-content style=\"fixed-case\">CN<\/jats:styled-content>, while <jats:styled-content style=\"fixed-case\">Dps<\/jats:styled-content> sequesters it, quelling <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>enton's reaction. We propose that disrupting intracellular iron trafficking is a common strategy employed by the immune system to kill microbes.<\/jats:p>","DOI":"10.1111\/mmi.12938","type":"journal-article","created":{"date-parts":[[2015,1,19]],"date-time":"2015-01-19T09:38:48Z","timestamp":1421660328000},"page":"349-367","source":"Crossref","is-referenced-by-count":19,"title":["Cyanide enhances hydrogen peroxide toxicity by recruiting endogenous iron to trigger catastrophic chromosomal fragmentation"],"prefix":"10.1111","volume":"96","author":[{"given":"Tulip","family":"Mahaseth","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Illinois at Urbana\u2010Champaign  Urbana IL 61801\u20103709 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Kuzminov","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Illinois at Urbana\u2010Champaign  Urbana IL 61801\u20103709 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