{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:01:58Z","timestamp":1791414118723,"version":"4.3.3"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,6,6]],"date-time":"2015-06-06T00:00:00Z","timestamp":1433548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R37 AI-042347"],"award-info":[{"award-number":["R37 AI-042347"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Summary<\/jats:title>The diarrheal pathogen V<\/jats:italic><\/jats:styled-content>ibrio cholerae<\/jats:italic> contains three gene clusters that encode chemotaxis\u2010related proteins, but only cluster II<\/jats:styled-content> appears to be required for chemotaxis. Here, we present the first characterization of V<\/jats:italic><\/jats:styled-content>.\u2009cholerae<\/jats:italic>'s \u2018cluster III\u2019 chemotaxis system. We found that cluster III<\/jats:styled-content> proteins assemble into foci at bacterial poles, like those formed by cluster II<\/jats:styled-content> proteins, but the two systems assemble independently and do not colocalize. Cluster III<\/jats:styled-content> proteins are expressed in vitro<\/jats:italic> during stationary phase and in conjunction with growth arrest linked to carbon starvation. This expression, as well as expression in vivo<\/jats:italic> in suckling rabbits, is dependent upon RpoS<\/jats:styled-content>. V<\/jats:italic><\/jats:styled-content>.\u2009cholerae<\/jats:italic>'s CAI<\/jats:styled-content>\u20101 quorum sensing (QS<\/jats:styled-content>) system is also required for cluster III<\/jats:styled-content> expression in stationary phase and modulates its expression in vivo<\/jats:italic>, but is not required for cluster III<\/jats:styled-content> expression in response to carbon starvation. Surprisingly, even though the CAI<\/jats:styled-content>\u20101 and AI<\/jats:styled-content>\u20102 QS<\/jats:styled-content> systems are thought to feed into the same signaling pathway, the AI<\/jats:styled-content>\u20102 system inhibited cluster III<\/jats:styled-content> gene expression, revealing that the outputs of the two QS<\/jats:styled-content> systems are not always the same. The distinctions between genetic determinants of cluster III<\/jats:styled-content> expression in vitro<\/jats:italic> and in vivo<\/jats:italic> highlight the distinctive nature of the in vivo<\/jats:italic> environment.<\/jats:p>","DOI":"10.1111\/mmi.13053","type":"journal-article","created":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T02:29:34Z","timestamp":1432002574000},"page":"660-675","source":"Crossref","is-referenced-by-count":31,"title":["RpoS<\/scp> and quorum sensing control expression and polar localization of V<\/i><\/scp>ibrio cholerae<\/i> chemotaxis cluster III<\/scp> proteins in vitro<\/i> and in vivo<\/i>"],"prefix":"10.1111","volume":"97","author":[{"given":"Simon","family":"Ringgaard","sequence":"first","affiliation":[{"name":"Department of Ecophysiology Max Planck Institute for Terrestrial Microbiology Marburg 35043 Germany"},{"name":"Division of Infectious Diseases Brigham and Women's Hospital Boston MA 02115 USA"},{"name":"Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy","family":"Hubbard","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Brigham and Women's Hospital Boston MA 02115 USA"},{"name":"Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Mandlik","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Brigham and Women's Hospital Boston MA 02115 USA"},{"name":"Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigid M.","family":"Davis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Brigham and Women's Hospital Boston MA 02115 USA"},{"name":"Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew K.","family":"Waldor","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Brigham and Women's Hospital Boston MA 02115 USA"},{"name":"Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA"},{"name":"Howard Hughes Medical Institute Brigham and Women's Hospital Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102946"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04489.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.08.039"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308052101"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1207"},{"key":"e_1_2_6_7_1","first-page":"4310","article-title":"Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive\u2010selection suicide vector","volume":"59","author":"Donnenberg M.S.","year":"1991","journal-title":"J Bacteriol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20195"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.6.1767-1771.2002"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752-0509-4-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043175"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35020000"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12887"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.24.8403-8410.2005"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02804-06"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku891"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456299"},{"key":"e_1_2_6_18_1","volume-title":"Brock Biology of Microorganisms","author":"Madigan M.T.","year":"2000"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.07.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.28867"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00829-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.6.2575-2583.1988"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004405"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-102108-134304"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020109"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908347106"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2061811"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315722111"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00047-10"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012427"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.178"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02044.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.2.301-319.2004"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1524"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608341104"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00295-8"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1770"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13053","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T06:33:11Z","timestamp":1696228391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,6]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/mmi.13053"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13053","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,6]]}}}