{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:28:44Z","timestamp":1789594124267,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["Ha1011\/11-1, Ha1011\/11-2"],"award-info":[{"award-number":["Ha1011\/11-1, Ha1011\/11-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Summary<\/jats:title>Beta\u2010lactam resistant clinical isolates of S<\/jats:italic><\/jats:styled-content>treptococcus pneumoniae<\/jats:italic> contain altered penicillin\u2010binding protein (PBP<\/jats:styled-content>) genes and occasionally an altered mur<\/jats:italic>M<\/jats:italic><\/jats:styled-content>, presumably products of interspecies gene transfer. MurM<\/jats:styled-content> and MurN<\/jats:styled-content> are responsible for the synthesis of branched lipid II<\/jats:styled-content>, substrate for the PBP<\/jats:styled-content> catalyzed transpeptidation reaction. Here we used the high\u2010level beta\u2010lactam resistant S<\/jats:italic><\/jats:styled-content>.\u2009oralis<\/jats:italic>\u2005U<\/jats:styled-content>o5 as donor in transformation experiments with the sensitive laboratory strain S<\/jats:italic><\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> R6 as recipient. Surprisingly, piperacillin\u2010resistant transformants contained no alterations in PBP<\/jats:styled-content> genes but carried mur<\/jats:italic>E<\/jats:italic><\/jats:styled-content>Uo5<\/jats:sub> encoding the UDP<\/jats:styled-content>\u2010N<\/jats:styled-content>\u2010acetylmuramyl tripeptide synthetase. Codons 83\u2013183 of mur<\/jats:italic>E<\/jats:italic><\/jats:styled-content>Uo5<\/jats:sub> were sufficient to confer the resistance phenotype. Moreover, the promoter of mur<\/jats:italic>E<\/jats:italic><\/jats:styled-content>Uo5<\/jats:sub>, which drives a twofold higher expression compared to that of S<\/jats:italic><\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> R6, could also confer increased resistance. Multiple independent transformations produced S<\/jats:italic><\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2005R<\/jats:styled-content>6 derivatives containing mur<\/jats:italic>E<\/jats:italic><\/jats:styled-content>Uo5<\/jats:sub>, pbp2x<\/jats:italic>Uo5<\/jats:sub>, pbp1a<\/jats:italic>Uo5<\/jats:sub> and pbp2b<\/jats:italic>Uo5<\/jats:sub>, but not mur<\/jats:italic>M<\/jats:italic><\/jats:styled-content>Uo5<\/jats:sub> sequences; however, the resistance level of the donor strain could not be reached. S<\/jats:italic><\/jats:styled-content>.\u2009oralis<\/jats:italic>\u2005U<\/jats:styled-content>o5 harbors an unusual mur<\/jats:italic>M<\/jats:italic><\/jats:styled-content>, and mur<\/jats:italic>N<\/jats:italic><\/jats:styled-content> is absent. Accordingly, the peptidoglycan of S<\/jats:italic><\/jats:styled-content>.\u2009oralis<\/jats:italic>\u2005U<\/jats:styled-content>o5 contained interpeptide bridges with one L<\/jats:styled-content>\u2010A<\/jats:styled-content>la residue only. The data suggest that resistance in S<\/jats:italic><\/jats:styled-content>.\u2009oralis<\/jats:italic>\u2005U<\/jats:styled-content>o5 is based on a complex interplay of distinct PBPs<\/jats:styled-content> and other enzymes involved in peptidoglycan biosynthesis.<\/jats:p>","DOI":"10.1111\/mmi.13070","type":"journal-article","created":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T02:55:42Z","timestamp":1432608942000},"page":"866-880","source":"Crossref","is-referenced-by-count":27,"title":["Transfer of penicillin resistance from S<\/i><\/scp>treptococcus oralis<\/i> to S<\/i><\/scp>treptococcus pneumoniae<\/i> identifies mur<\/i>E<\/i><\/scp> as resistance determinant"],"prefix":"10.1111","volume":"97","author":[{"given":"Katya","family":"Todorova","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Kaiserslautern Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Maurer","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Kaiserslautern Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Rieger","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Kaiserslautern Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Kaiserslautern Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhat Khai","family":"Bui","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Cell Biology Institute for Cell and Molecular Biosciences Newcastle University Newcastle upon Tyne NE2\u20094AX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Gray","sequence":"additional","affiliation":[{"name":"Institute for Cell and Molecular Biosciences Pinnacle Laboratory Newcastle University Newcastle upon Tyne NE2\u20094HH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waldemar","family":"Vollmer","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Cell Biology Institute for Cell and Molecular Biosciences Newcastle University Newcastle upon Tyne NE2\u20094AX UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regine","family":"Hakenbeck","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Kaiserslautern Kaiserslautern 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